Combinatorial interaction of light-responsive elements plays a critical role in determining the response characteristics of light-regulated promoters in Arabidopsis
Combinatorial interaction of light-responsive elements plays a critical role in determining the response characteristics of light-regulated promoters in Arabidopsis
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DOI：
10.1046/j.1365-313x.1998.00180.x
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发表时间：
1998-07-01
期刊：
影响因子：
7.2
通讯作者：
Wei, N
中科院分区：
文献类型：
--
作者：
Chattopadhyay, S;Puente, P;Wei, N
我们研究了PhyA、PhyB和CRY1光感受器及其下游的光信号成分COP1和DET1在含有合成光响应元件(LRE)的启动子的高辐射光控活性中的作用。成对的LRE启动子能够通过多个感光器对广谱的光做出反应，而光诱导的单个LRE启动子主要对特定波长的光做出反应。此外，我们的结果表明，Cry1参与了PhyB介导的G-GATA/NOS101启动子的红光诱导，并且Cry1和PhyB都是红光或蓝光有效抑制GT1/NOS101启动子所必需的。在介导GT1-GATA/NOS101启动子光激活过程中，PhyA和PhyB之间存在相互作用。此外，我们的数据表明COP1和DET1在黑暗中只对成对的LRE启动子起负调控作用，而不是单个LRE启动子。根据这些结果，我们得出结论，LRE的组合相互作用在决定光响应启动子受关键细胞调节因子调控和对不同光环境做出反应的能力方面是必不可少的。
We have studied the roles of PhyA, PhyB and CRY1 photoreceptors and the downstream light-signaling components, COP1 and DET1, in mediating high-irradiance light-controlled activity of promoters containing synthetic light-responsive elements (LRE). Promoters with paired LREs were able to respond to a wide spectrum of light through multiple photoreceptors, while the light-inducible single LRE promoters primarily responded to a specific wavelength of light. In addition, our results indicate that Cry1 is involved in PhyB-mediated red-light induction of the G-GATA/NOS101 promoter, and that both Cry1 and PhyB are required for effective repression of the GT1/NOS101 promoter by red or blue light. An interaction between PhyA and PhyB in mediating GT1-GATA/NOS101 promoter light activation was also observed. Furthermore, our data indicate that COP1 and DET1 exert negative control in the dark only on paired LRE promoters but not single LRE promoters. From these results, we conclude that the combinatorial interaction of LREs is essential in determining the ability of light-responsive promoters to be modulated by crucial cellular regulators and to respond to diverse light environments.