TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats.
TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats.
复制标题
TSP-1促进Thy-1肾炎大鼠肾小球系膜细胞增殖和细胞外基质分泌
DOI：
10.1016/s1674-8301(11)60053-5
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发表时间：
2011-11
影响因子：
2.3
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Qiu W;Li Y;Zhou J;Zhao C;Zhang J;Shan K;Zhao D;Wang Y
类似人类系膜增生性肾炎的Thy-1肾炎(Thy-1N)大鼠的肾小球系膜细胞(GMC)增殖和细胞外基质(ECM)的分泌已被探索多年，但GMC增殖和ECM产生的分子机制尚不清楚。我们前期的研究表明，TSP-1基因参与了亚溶血C5b-9诱导的大鼠GMC增殖和细胞外基质的合成。本研究用TSP-1小发夹状RNA技术检测了TSP-1基因在Thy-1N大鼠肾小球系膜细胞增殖、细胞外基质产生和尿蛋白分泌中的作用。结果表明，TSP-1基因沉默可抑制Thy-1N大鼠肾小球系膜细胞增殖(P<；0.01)、细胞外基质分泌(P<；0.01)和尿蛋白分泌(P<；0.05)。综上所述，本研究结果提示TSP-1基因表达是Thy-1N大鼠GMC增殖和ECM产生所必需的。
The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro. In the present study, the roles of the TSP-1 gene in GMC proliferation, ECM production, and urinary protein secretion in Thy-1N rats were determined by using TSP-1 small hairpin RNA, and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P < 0.01) and ECM secretion (P < 0.01) as well as urinary protein secretion (P < 0.05) in Thy-1N rats. Together, the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-1N rats.