Isolation of Candidatus Bartonella rousetti and Other Bat-associated Bartonellae from Bats and Their Flies in Zambia
Isolation of Candidatus Bartonella rousetti and Other Bat-associated Bartonellae from Bats and Their Flies in Zambia
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DOI：
10.3390/pathogens9060469
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发表时间：
2020-06-01
期刊：
影响因子：
3.7
通讯作者：
Sugimoto, Chihiro
中科院分区：
文献类型：
--
作者：
Qiu, Yongjin;Kajihara, Masahiro;Sugimoto, Chihiro
蝙蝠相关的巴尔通体，包括Bartonella mayotimonensis和Bartonella rousetti，最近被确定为新兴和潜在的人畜共患病的代理人，分别。然而，在赞比亚没有与蝙蝠相关的bartonalgia的报告。因此，我们的目的是分离和表征巴尔通体属。从赞比亚捕获的蝙蝠和蝙蝠蝇中提取的DNA。总体而言，巴尔通体属（Bartonella spp.）从36只蝙蝠中的6只（16.7%）中分离出巴尔通体DNA，而在19只蝙蝠蝇中的9只（47.3%）中检测到巴尔通体DNA。随后使用五个不同基因的序列表征显示，从埃及果蝠（Rousettus aegyptiacus）获得的三个分离株为Ca。B。罗塞蒂。从食虫蝙蝠（Macronycteris vittatus）获得的分离物被分为两个以前未分类的蝙蝠相关bartonerum。从9个病原体阳性蝙蝠苍蝇的巴尔通体gltA序列的6种基因型的系统发育分析显示，3种基因型属于相同的分支，蝙蝠相关的巴尔通体，包括Ca。B。罗塞蒂。其他三种基因型代表节肢动物相关的bartonopathy，这是以前只从体外寄生虫分离。我们证明了Ca。B。rousetti是维持蝙蝠之间（R.埃及伊蚊和蝙蝠蝇。巴尔通体属的持续监测。在蝙蝠和人类血清学调查在非洲是必要的，以评估公共卫生的重要性，蝙蝠相关bartonastrophic。
Bat-associated bartonellae, including Bartonella mayotimonensis and Candidatus Bartonella rousetti, were recently identified as emerging and potential zoonotic agents, respectively. However, there is no report of bat-associated bartonellae in Zambia. Thus, we aimed to isolate and characterize Bartonella spp. from bats and bat flies captured in Zambia by culturing and PCR. Overall, Bartonella spp. were isolated from six out of 36 bats (16.7%), while Bartonella DNA was detected in nine out of 19 bat flies (47.3%). Subsequent characterization using a sequence of five different genes revealed that three isolates obtained from Egyptian fruit bats (Rousettus aegyptiacus) were Ca. B. rousetti. The isolates obtained from insectivorous bats (Macronycteris vittatus) were divided into two previously unclassified bat-associated bartonellae. A phylogenetic analysis of the six genotypes of Bartonella gltA sequences from nine pathogen-positive bat flies revealed that three genotypes belonged to the same clades as bat-associated bartonellae, including Ca. B. rousetti. The other three genotypes represented arthropod-associated bartonellae, which have previously been isolated only from ectoparasites. We demonstrated that Ca. B. rousetti is maintained between bats (R. aegyptiacus) and bat flies in Zambia. Continuous surveillance of Bartonella spp. in bats and serological surveys in humans in Africa are warranted to evaluate the public health importance of bat-associated bartonellae.