Multimodal neural recordings with Neuro-FITM uncover diverse patterns of cortical-hippocampal interactions.
Multimodal neural recordings with Neuro-FITM uncover diverse patterns of cortical-hippocampal interactions.
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Neuro-FITM的多模态神经记录揭示了皮质-海马相互作用的不同模式。
DOI：
10.1038/s41593-021-00841-5
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发表时间：
2021-06
影响因子：
25
通讯作者：
Kuzum D
中科院分区：
文献类型：
--
作者：
Liu X;Ren C;Lu Y;Liu Y;Kim JH;Leutgeb S;Komiyama T;Kuzum D
许多认知过程需要新皮层和海马体之间的通信。然而，大规模皮质动力学和海马活动之间的协调尚未得到很好的理解，部分原因是难以同时记录这些区域。在这里，我们开发了一个灵活的，可插入的，和透明的微电极阵列（'Neuro-FITM'），使调查皮层海马协调在海马尖波波纹（SWR）。Neuro-FITM的灵活性和透明度允许在宽场钙成像期间同时记录局部场电位和海马神经尖峰。这些实验表明，不同的皮层活动模式伴随着SWRs，在大多数情况下，皮层激活海马SWRs之前。我们证明，在SWRs，不同的海马神经细胞群的活动与不同的皮质活动模式。这些结果表明，海马和大规模的皮质活动相互作用的选择性和多样性的方式在SWR潜在的各种认知功能。我们的技术可以广泛应用于皮质和其他皮质下结构之间的相互作用的全面调查。
Many cognitive processes require communication between neocortex and hippocampus. However, coordinations between large-scale cortical dynamics and hippocampal activity are not well-understood, partially due to the difficulty to simultaneously record from those regions. Here we developed a Flexible, Insertable, and Transparent Microelectrode array (‘Neuro-FITM’) that enables investigation of cortical-hippocampal coordinations during hippocampal sharp-wave ripples (SWRs). Flexibility and transparency of Neuro-FITM allow simultaneous recordings of local field potentials and neural spiking from hippocampus during wide-field calcium imaging. These experiments revealed that diverse cortical activity patterns accompanied SWRs, and in most cases, cortical activation preceded hippocampal SWRs. We demonstrated that, during SWRs, different hippocampal neural population activity was associated with distinct cortical activity patterns. These results suggest that hippocampus and large-scale cortical activity interact in a selective and diverse manner during SWRs underlying various cognitive functions. Our technology can be broadly applied to comprehensive investigations of interactions between cortex and other subcortical structures.