Inactivation of a basement membrane component responsible for cell proliferation but not for cell attachment.
Inactivation of a basement membrane component responsible for cell proliferation but not for cell attachment.
复制标题
负责细胞增殖但不负责细胞附着的基底膜成分失活。
DOI：
10.1016/0014-4827(82)90149-5
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发表时间：
1982
影响因子：
3.7
通讯作者：
Gospodarowicz,D
中科院分区：
文献类型：
--
作者：
Greenburg,G;Gospodarowicz,D
分析了各种化学、酶和热处理对培养的牛角膜内皮细胞产生的基底膜（BM）支持细胞增殖与细胞附着的能力的影响。碱性处理（pH 13.9），这是已知的蛋白质和碳水化合物部分之间的O-糖苷键裂解蛋白聚糖或糖肽导致BM包被的培养皿，以支持细胞生长的能力下降了97%。同样，用4 M盐酸胍处理，可提取高达80%的糖胺聚糖，导致细胞生长减少92%。亚硝酸（HNO 2）处理，降解硫酸乙酰肝素，并释放硫酸化二糖和非硫酸化寡糖，也减少了90%的细胞生长。用特异性蛋白酶、胶原酶或糖苷酶处理BM不会明显降低BM支持细胞生长的能力。因此，本研究中测试的酶所使用的任何底物是否能在细胞增殖中发挥直接作用值得怀疑。碱处理骨髓，虽然会破坏其支持细胞生长的能力，但不会改变细胞附着在其上的能力。相反，这种处理甚至会略微改善这种能力。相反，BM的热处理（70 °C）不会降低细胞在BM上生长的能力，但会强烈降低细胞附着在BM上的能力。因此，BM的热处理而不是碱处理可能优先影响附着因子，而不是生长促进因子，并且这些因子是独立的实体。
The effect of various chemical, enzymatic, and heat treatments on the ability of basement membrane (BM) produced by cultured bovine corneal endothelial cells to support cell proliferation vs cell attachment has been analysed. Alkaline treatment (pH 13.9), which is known to cleave proteoglycans or glycopeptides at the O-glycosidic bond between the protein and carbohydrate moieties resulted in a 97% decrease in the ability of BM-coated dishes to support cell growth. Likewise, treatment with 4 M guanidine-HCl, which extracts up to 80% of the glycosaminoglycan, caused a 92% reduction in cell growth. Nitrous acid (HNO2) treatment, which degrades heparan sulphate and releases both sulphated disaccharide and non-sulphated oligosaccharides, also reduced cell growth by 90%. Treatment of the BM with specific protease, collagenase, or glycosidase did not appreciably decrease the ability of the BM to support cell growth. It is therefore doubtful that any of the substrates used for the enzymes tested in the present studies could play a direct role in cell proliferation. Alkali treatment of BM, which abolishes its ability to support cell growth, does not alter the cells' ability to attach to it. On the contrary, such treatment even improves it slightly. Conversely, heat treatment of BM (70 °C), which does not reduce the ability of the cells to grow on BM, strongly reduces their ability to attach to it. It is therefore likely that heat treatment, but not alkali treatment of BM preferentially affects attachment factors, but not growth-promoting factors, and that these factors are separate entities.