REVERSION ANALYSIS OF [PSI-] MUTATIONS IN SACCHAROMYCES-CEREVISIAE
REVERSION ANALYSIS OF [PSI-] MUTATIONS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1017/s0016672300020140
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发表时间：
1981-01-01
影响因子：
1.5
通讯作者：
COX, BS
中科院分区：
文献类型：
--
作者：
LUND, PM;COX, BS
[psi]是酵母S.在用多种试剂处理后分离酿酒酵母，所述试剂包括常规诱变剂和许多在高频率下引起[psi]损失的化合物，即，甲醇、KCl、二甲亚砜[DMSO]和盐酸胍[GuHCl]。在psi-]突变体中，抑制子SUQ 5不抑制赭石突变如ade2.1。[psi-]突变体的回复分析显示了3类：一类产生[psi-]突变的试剂，其可以容易地回复为[psi+]（甲醇、KCl和DMSO属于该类）;不能显示回复GuHCl的试剂，以及产生可回复和明显不可回复的[psi-]突变的常规诱变剂。GuHCl导致[psi]因子缺失或丢失。甲醇可以引起例如启动子的状态改变，并且KCl可以选择或诱导[psi+]菌株的低拷贝数变体。DMSO可能参与[psi]的调节。
Mutants of [psi], a cytoplasmically inherited factor in the yeast S. cerevisiae were isolated after treatment with a variety of agents including conventional mutagens and a number of compounds which cause loss of [psi] at high frequencies, i.e., methanol, KCl, dimethyl sulfoxide [DMSO] and guanidine hydrochloride [GuHCl]. In psi-] mutants, the suppressor SUQ5 does not suppress ochre mutations such as ade2.1. Reversion analysis of the [psi-] mutants revealed 3 classes: a class of agents producing [psi-] mutations which could readily revert to [psi+] (methanol, KCl and DMSO belong to this class); those which could not be shown to revert GuHCl, and the conventional mutagens which produced both revertible and apparently non-revertible [psi-] mutations. GuHCl causes a deletion or loss of the [psi] factor. Methanol may cause an alteration of state for example, of a promoter, and KCl may be selecting or inducing low copy number variants of [psi+] strains. It is possible that DMSO may be involved in regulation of [psi].