Growth and carbon content of three different-sized diazotrophic cyanobacteria observed in the subtropical North Pacific
Growth and carbon content of three different-sized diazotrophic cyanobacteria observed in the subtropical North Pacific
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DOI：
10.1111/j.1529-8817.2008.00581.x
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发表时间：
2008-10-01
影响因子：
2.9
通讯作者：
Zehr, Jonathan P.
中科院分区：
文献类型：
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作者：
Goebel, Nicole L.;Edwards, Christopher A.;Zehr, Jonathan P.
为了开发模拟开放海洋重氮营养生长的工具，我们测定了北太平洋副热带ALOHA站（长期寡营养栖息地评估）中常见的三种重氮营养蓝藻的最大生长速率和碳含量：丝状非异囊型毛菌群和单细胞群A和b。菌株IMS101和沃特伯里鳄（Crocosphaera watsonii J. Waterbury）菌株WH8501在实验室进行测定。最大生长率（0.51 +/- 0.09 vs. 0.49 +/- 0.17 d(-1)）、半光饱和度（73 +/- 29 vs. 66 +/- 37 mu mol量子）的拟合参数（+/- CI）之间无显著差异。(2)。S(-1))，光抑制(0和0.00043 +/- 0.00087 [mu mol量子]。(2)。(1)](1))。trichodesium和Crocosphaera培养物的最大生长速率和碳含量符合已发表的异速生长关系，表明这些关系适用于海洋重氮营养微生物。这种一致促进了异速生长模型的使用，以近似未知参数的最大生长率（0.77 d(-1)）和碳含量（480 fg C）。μ m(-3))为未培养的单细胞A族蓝藻。利用荧光活化细胞分选和实时定量PCR技术，从北太平洋的样本中鉴定了A群的大小。了解这些生物的生长和碳含量特性有助于将不同类型的蓝藻纳入建模工作，旨在评估丝状和单细胞重氮营养体对开放海洋中碳和氮循环的相对重要性。
To develop tools for modeling diazotrophic growth in the open ocean, we determined the maximum growth rate and carbon content for three diazotrophic cyanobacteria commonly observed at Station ALOHA (A Long-term Oligotrophic Habitat Assessment) in the subtropical North Pacific: filamentous nonheterocyst-forming Trichodesmium and unicellular Groups A and B. Growth-irradiance responses of Trichodesmium erythraeum Ehrenb. strain IMS101 and Crocosphaera watsonii J. Waterbury strain WH8501 were measured in the laboratory. No significant differences were detected between their fitted parameters (+/- CI) for maximum growth rate (0.51 +/- 0.09 vs. 0.49 +/- 0.17 d(-1)), half-light saturation (73 +/- 29 vs. 66 +/- 37 mu mol quanta . m(-2) . s(-1)), and photoinhibition (0 and 0.00043 +/- 0.00087 [mu mol quanta . m(-2) . s(-1)](-1)). Maximum growth rates and carbon contents of Trichodesmium and Crocosphaera cultures conformed to published allometric relationships, demonstrating that these relationships apply to oceanic diazotrophic microorganisms. This agreement promoted the use of allometric models to approximate unknown parameters of maximum growth rate (0.77 d(-1)) and carbon content (480 fg C . mu m(-3)) for the uncultivated, unicellular Group A cyanobacteria. The size of Group A was characterized from samples from the North Pacific Ocean using fluorescence-activated cell sorting and real-time quantitative PCR techniques. Knowledge of growth and carbon content properties of these organisms facilitates the incorporation of different types of cyanobacteria in modeling efforts aimed at assessing the relative importance of filamentous and unicellular diazotrophs to carbon and nitrogen cycling in the open ocean.