Gastric mucosal tyrosine kinase activity during aging and its relationship to cell proliferation in rats.
Gastric mucosal tyrosine kinase activity during aging and its relationship to cell proliferation in rats.
复制标题
大鼠衰老过程中胃粘膜酪氨酸激酶活性及其与细胞增殖的关系。
DOI：
10.1016/0304-4165(88)90043-8
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Elizabeth A. Edgerton
中科院分区：
文献类型：
--
作者：
Adhip P. Nandi Manjmdar;Adhip P. Nandi Manjmdar;Elizabeth A. Edgerton
研究了胃粘膜中酪氨酸激酶活性和细胞增殖活性之间的关系。为了进行比较，还包括肝脏和胰腺。使用 2 个月、14 个月和 22 个月大的雄性 Fischer-344 大鼠组。使用合成聚合物 Glu-Tyr (4:1) 作为底物测定膜组分 (30 000 × gpellet) 中的酪氨酸激酶活性。通过测量20000×g上清液中的鸟氨酸脱羧酶来评估细胞增殖活性。在所有年龄组中，胃粘膜酪氨酸激酶活性比肝脏或胰腺高 10-20 倍。此外，22 月龄大鼠的胃粘膜酪氨酸激酶活性比 2 月龄和 14 月龄大鼠的胃粘膜酪氨酸激酶活性高 35-70%。胃粘膜鸟氨酸脱羧酶活性也遵循与酪氨酸激酶基本相同的模式，在22个月大的大鼠中观察到最高活性。 22月龄大鼠胃粘膜增殖活性的增加也与粘膜蛋白酪氨酸磷酸化的增加有关，其表观Mrof为53 000。胰腺中出现相反的现象，其增殖活性最低。结论是，胃粘膜增殖活性的年龄相关变化伴随着酪氨酸激酶活性的平行变化。 53 kDa 膜蛋白的酪氨酸磷酸化可能在细胞增殖的调节中发挥作用。
The relationship between tyrosin kinase activity and cellular proliferative activity was investigated in the gastric mucosa. For the purpose of comparison, the liver and the pancreas were also included. Groups of 2-, 14- and 22-month-old male Fischer-344 rats were used. Tyrosine kinase activity was determined in the membrane fraction (30 000 ×gpellet) utilizing a synthetic polymer, Glu-Tyr (4:1), as substrate. Cellular proliferative activity was assessed by measuring ornithine decarboxylase in the 20 000 ×gsupernatant. In all age groups, gastric mucosal tyrosine kinase activity was found to be 10–20-fold higher than in the liver or pancreas. In addition, gastric mucosal tyrosine kinase activity in 22-month-old rats was 35–70% higher than in their 2- and 14-month-old counterparts. Gastric mucosal ornithine decarboxylase activity also followed essentially the same pattern as that the tyrosine kinase in that the highest activity was observed in 22-month-old rats. Increased gastric mucosal proliferative activity in 22-month-old rats was also associated with increased tyrosine-phosphorylation of a mucosal membrane protein with an apparentMrof 53 000. An opposite phenomenon occurred in the pancreas whose proliferative activity was found to be the lowest. It is concluded that the age-associated changes in gastric mucosal proliferative activity are accompanied by parallel alterations in tyrosine kinase activity. Tyrosine-phosphorylation of a 53 kDa membrane protein may play a role in the regulation of cell proliferation.
不同生理状态下小鼠乳腺表皮生长因子受体水平。
影响因子：
4.8
作者：
Edery,M;Pang,K;Larson,L;Colosi,T;Nandi,S
通讯作者：
Nandi,S
鼠 Leydig 肿瘤细胞中蛋白酪氨酸激酶活性的表征。
DOI：
10.1016/0167-4889(86)90136-9
发表时间：
1986
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Dangott,LJ;Puett,D;Melner,MH
通讯作者：
Melner,MH