Effects of gibberellin on growth and development of rose 'Carola'.
Effects of gibberellin on growth and development of rose 'Carola'.
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DOI：
10.16420/j.issn.0513-353x.2018-0462
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Jin, W. M.
中科院分区：
文献类型：
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作者：
Li, Mao-fu;Yang, Yuan;Jin, W. M.
本研究将不同浓度的赤霉素（GA3）施用于玫瑰花冠的枝条上，并以喷水作为对照。记录初花日期、花开放日期和花谢日期，并测量盛花期的花枝长度、花梗长度、株高和叶片纵横比，以揭示GA3对玫瑰‘卡罗拉’生长发育的影响。 GA3处理浓度为300 mg.L-1时,月季平均长度最长,叶片长径比最大;浓度为300和400 mg.L-1处理时,花梗平均长度最长;浓度为500 mg.L-1时,平均株高最高。在喷洒GA3 300 mg.L-1条件下，RT-PCR结果表明，施用GA3上调了RhGA2ox、RhGA20ox、RhGA3ox和RhGID1基因的表达，这些基因可能参与GAs代谢和激素信号传导的调节。此外，这些变化可能间接诱导生长素合成相关基因的表达，导致细胞伸长和细胞体积增加，进而促进玫瑰植物株高和叶长的增加。对相关开花相关基因RhAP1、RhKSN和RhSOC1的研究表明，施用GA3后，RhAP1、RhFT和RhSOC1基因表达水平先升高后降低，与对照相比变化较大。开花基因RhFT的高表达可能是GA3处理导致花期提前的原因之一；此外，RhKSN的高表达可能是花期延长的原因之一。
In this study, different concentrations of gibberellin (GA3) were applied to the branch of the rose, 'Corolla', and the water spraying was used as the control. The initial flower date, the date of flower opening, and the date of flower withering were recorded and the length of flower branches, flower stalks, plant heights and the ratio of leaf longitudinal and horizontal were measured at full-bloom stage, in order to reveal the effects of GA3 on the growth and development of rose 'Carola'. When the treatment concentration of GA3 was 300 mg.L-1, the average length of the rose was the longest and the aspect ratio of the leaf was the largest;the treatment with the concentration of 300 and 400 mg.L-1 had the longest average length of the flower stalks;the concentration 500 mg.L-1 had the highest average plant height. Under the spraying condition of GA3 300 mg.L-1, RT-PCR results demonstrated that the application of GA3 up-regulated the expression of RhGA2ox, RhGA20ox, RhGA3ox, and RhGID1 genes, which might participate in the regulation of GAs metabolism and hormone signaling. Furthermore, the changes might indirectly induce the expression of genes related to auxin synthesis, leading to cell elongation and cell volume increase, which in turn promoted the increase of the plant height and the leaf length in rose plants. The investigation of related flowering-related genes, RhAP1, RhKSN and RhSOC1 showed that the expression pattern of RhAP1, RhFT and RhSOC1 gene expression levels were first to increase and then decrease after the application of GA3, and changed greatly, compared with the control. The high expression of the flowering gene, RhFT may be one of the causes of the earlier date for flowering due to GA3 treatment;in addition, the high expression of RhKSN may be one of the reasons for the longer period of flowering.