A dominant negative Raf-1 mutant prevents v-Src-induced transformation.
A dominant negative Raf-1 mutant prevents v-Src-induced transformation.
复制标题
显性失活 Raf-1 突变体可阻止 v-Src 诱导的转化。
DOI：
10.1006/bbrc.1993.1510
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发表时间：
1993
影响因子：
3.1
通讯作者：
Foster,DA
中科院分区：
文献类型：
--
作者：
Qureshi,SA;Joseph,CK;Hendrickson,M;Song,J;Gupta,R;Bruder,J;Rapp,U;Foster,DA
将表达Raf-1显性负突变体的载体稳定导入表达v-Src温度敏感衍生物的BALB/c 3 T3细胞中。在这些细胞中检测到高水平的Raf-mutant。Raf-1突变体的表达阻断了v-Src诱导的转化，如通过回复到平坦的非转化形态和不能在软琼脂中形成菌落所确定的。用缺乏突变体Raf-1的亲本载体转染的细胞可以被v-Src转化。这些数据表明，由Raf-1介导的v-Src激活的细胞内信号是v-Src转化所必需的。
A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src.