Endothelial cell and platelet bioenergetics: effect of glucose and nutrient composition.
Endothelial cell and platelet bioenergetics: effect of glucose and nutrient composition.
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DOI：
10.1371/journal.pone.0039430
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sivitz WI
中科院分区：
文献类型：
--
作者：
Fink BD;Herlein JA;O'Malley Y;Sivitz WI
有人提出，不依赖胰岛素摄取葡萄糖的细胞，当暴露于高葡萄糖或其他营养浓度时，会表现出线粒体底物氧化增强，从而增强电位并产生活性氧（ROS）；这种模式可能导致糖尿病血管并发症。在这里，我们将牛主动脉内皮 (BAE) 细胞和人血小板暴露于不同浓度的葡萄糖和脂肪酸中。然后，我们使用最新可用的细胞外氧和质子通量分析仪形式的技术检查了耗氧量和酸化率。将汇合的 BAE 细胞急性或过夜暴露于 5.5 至 25 mM 的葡萄糖浓度下，不会增强或改变基础条件下、ATP 合成过程中或非偶联条件下的耗氧率 (OCR)。葡萄糖也不会改变亚汇合细胞、暴露于低血清的细胞或添加丙酮酸处理的细胞中的 OCR。同样，过夜暴露于不同饱和度的脂肪酸也没有这样的影响。 BAE 细胞过夜暴露于低葡萄糖浓度会降低最大非耦合呼吸，但不会降低基础或 ATP 相关耗氧量。标记的葡萄糖氧化成 CO2 的情况有所增加，但在高葡萄糖暴露后仅略有增加，而油酸氧化成 CO2 的情况则有所减少。过夜接触亚麻酸，但不接触油酸或亚油酸，会增加细胞外酸化，这与糖酵解代谢的增强一致。我们无法检测到暴露于高培养基葡萄糖的 BAE 细胞活性氧 (ROS) 产量的增加。与 BAE 细胞一样，将人类血小板暴露于葡萄糖不会增加耗氧量。与 BAE 细胞相反，血小板线粒体表现出较低的呼吸储备能力（超出基础代谢所需的能力）。我们的数据不支持暴露于高葡萄糖或脂肪酸会加速内皮细胞或血小板中线粒体氧化代谢的概念。
It has been suggested that cells that are independent of insulin for glucose uptake, when exposed to high glucose or other nutrient concentrations, manifest enhanced mitochondrial substrate oxidation with consequent enhanced potential and generation of reactive oxygen species (ROS); a paradigm that could predispose to vascular complications of diabetes. Here we exposed bovine aortic endothelial (BAE) cells and human platelets to variable glucose and fatty acid concentrations. We then examined oxygen consumption and acidification rates using recently available technology in the form of an extracellular oxygen and proton flux analyzer. Acute or overnight exposure of confluent BAE cells to glucose concentrations from 5.5 to 25 mM did not enhance or change the rate of oxygen consumption (OCR) under basal conditions, during ATP synthesis, or under uncoupled conditions. Glucose also did not alter OCR in sub-confluent cells, in cells exposed to low serum, or in cells treated with added pyruvate. Likewise, overnight exposure to fatty acids of varying saturation had no such effects. Overnight exposure of BAE cells to low glucose concentration decreased maximal uncoupled respiration, but not basal or ATP related oxygen consumption. Labeled glucose oxidation to CO2 increased, but only marginally after high glucose exposure while oleate oxidation to CO2 decreased. Overnight exposure to linolenic acid, but not oleic or linoleic acid increased extracellular acidification consistent with enhanced glycolytic metabolism. We were unable to detect an increase in production of reactive oxygen species (ROS) from BAE cells exposed to high medium glucose. Like BAE cells, exposure of human platelets to glucose did not increase oxygen consumption. As opposed to BAE cells, platelet mitochondria demonstrate less respiratory reserve capacity (beyond that needed for basal metabolism). Our data do not support the concept that exposure to high glucose or fatty acids accelerates mitochondrial oxidative metabolism in endothelial cells or platelets.
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影响因子：
7.4
作者：
Dranka, Brian P.;Hill, Bradford G.;Darley-Usmar, Victor M.
通讯作者：
Darley-Usmar, Victor M.
影响因子：
2.9
作者：
Bulteau, AL;Ikeda-Saito, M;Szweda, LI
通讯作者：
Szweda, LI
影响因子：
5.5
作者：
Fink, B;Laude, K;Dikalov, S
通讯作者：
Dikalov, S
DOI：
10.1111/j.1582-4934.2003.tb00202.x
发表时间：
2003-01-01
影响因子：
5.3
作者：
Liu, Y;Song, DX;Zuo, J
通讯作者：
Zuo, J
影响因子：
3.3
作者：
Paramo, B.;Hernandez-Fonseca, K.;Massieu, L.
通讯作者：
Massieu, L.