miR-185 and miR-133b deregulation is associated with overall survival and metastasis in colorectal cancer
miR-185 and miR-133b deregulation is associated with overall survival and metastasis in colorectal cancer
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DOI：
10.3892/ijo.2011.1043
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发表时间：
2011-08-01
影响因子：
5.2
通讯作者：
Lui, Weng-Onn
中科院分区：
文献类型：
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作者：
Akcakaya, Pinar;Ekelund, Susanne;Lui, Weng-Onn
结直肠癌（CRC）是最常见和致命的癌症形式之一。尽管治疗方法有所改进，但术后复发和转移仍然是延长患者术后生存期的主要问题。这突出了寻找用于结直肠癌患者分类和治疗分层的生物标志物的需要。在这项研究中，我们应用基于SYBR-绿色定量PCR的阵列方法来筛选短生存期（< 50个月，范围10-33个月）和长生存期（>= 50个月，范围50-152个月）患者之间差异表达的miRNA。通过TaqMan定量PCR在50名CRC患者的队列中验证所选择的候选预后miRNA。我们发现，miR-185的高表达和miR-133 b的低表达与结直肠癌的生存率（分别为p=0.001和0.028）和转移（分别为p=0.007和0.036）相关。我们的研究结果表明这些miRNAs对预测手术后临床结果具有潜在的预后价值。
Colorectal cancer (CRC) is one of the most common and deadly forms of cancer. Despite improved treatment modalities, post-operative recurrence and metastasis remain the major problems for extending patient survival after surgery. This highlights the need to search for biomarkers for prognostication and treatment stratification of colorectal cancer patients. In this study, we applied the SYBR-green quantitative PCR-based array approach to screen for differentially expressed miRNAs between patients with short (< 50 months, range 10-33 months) and long survival (>= 50 months, range 50-152 months). The selected candidate prognostic miRNAs were validated in a cohort of 50 CRC patients by TaqMan quantitative PCR. We found that high expression of miR-185 and low expression of miR-133b were correlated with poor survival (p=0.001 and 0.028, respectively) and metastasis (p=0.007 and 0.036, respectively) in colorectal cancer. Our findings suggest the potential prognostic values of these miRNAs for predicting clinical outcome after surgery.