Comparison of the Interactions of Fibrinogen and Soluble Fibrin with Washed Rabbit Platelets Stimulated with ADP
Comparison of the Interactions of Fibrinogen and Soluble Fibrin with Washed Rabbit Platelets Stimulated with ADP
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纤维蛋白原和可溶性纤维蛋白与 ADP 刺激的洗涤兔血小板相互作用的比较
DOI：
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发表时间：
1985
影响因子：
6.7
通讯作者：
J. Mustard
中科院分区：
文献类型：
--
作者：
E. Harfenist;M. Packham;J. Mustard
由于在血管壁损伤部位形成的纤维蛋白参与血小板聚集体或血栓的形成和稳定，我们研究了纤维蛋白与兔血小板的反应。用纤维蛋白聚合抑制剂Gly-Pro-Arg-Pro制备可溶性纤维蛋白。单独使用纤维蛋白原并不引起洗涤血小板的聚集，但加入ADP引起的聚集和解聚与在纤维蛋白原存在下观察到的相同。125 I-纤维蛋白与ADP刺激的血小板的特异性结合类似于125 I-纤维蛋白原，但125 I-纤维蛋白不解离，即使在高浓度的腺苷三磷酸双磷酸酶的存在下。观察到125 I-纤维蛋白的高度非特异性结合，与聚集无关。EDTA，前列腺素E1（PGE 1）和磷酸肌酸/磷酸肌酸激酶阻止ADP诱导的聚集在纤维蛋白的存在下，并引起快速解聚后加入ADP。当在ADP之前加入时，它们也抑制125 I-纤维蛋白结合，并且EDTA或PGE 1引起结合的125 I-纤维蛋白的部分解离。在体内，纤维蛋白可以与刺激的血小板结合，凝结，形成凝胶，并与血浆、血小板聚集体和暴露的内皮下的组分相互作用。
Summary Because fibrin, formed at a site of vessel wall injury, is involved in the formation and stabilization of a platelet aggregate or thrombus, we have studied reactions of fibrin with rabbit platelets. Gly-Pro-Arg-Pro, an inhibitor of fibrin polymerization, was used to prepare soluble fibrin. Fibrin alone did not cause aggregation of washed platelets, but addition of ADP caused aggregation and deaggregation identical to those observed in the presence of fibrinogen. Specific binding of 125I-fibrin to ADP- stimulated platelets was similar to that of 125I-fibrinogen, but 125I-fibrin did not dissociate, even in the presence of high concentrations of apyrase. High non-specific binding of 125I-fibrin was observed that was not associated with aggregation. EDTA, prostaglandin E1 (PGE1) and creatine phosphate/creatine phos- phokinase prevented ADP-induced aggregation in the presence of fibrin and caused rapid deaggregation when added after ADP. They also inhibited 125I-fibrin binding when added before ADP, and EDTA or PGE1 caused partial dissociation of bound 125I-fibrin. In vivo, fibrin may bind to stimulated platelets, polymerize, form a gel, and interact with components of the plasma, the platelet aggregate, and the exposed subendothelium.
通过蛋白水解酶暴露人血小板上的纤维蛋白原受体。
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Niewiarowski,S;Budzynski,AZ;Morinelli,TA;Brudzynski,TM;Stewart,GJ
通讯作者：
Stewart,GJ