Spin-labeled psoralen probes for the study of DNA dynamics.
Spin-labeled psoralen probes for the study of DNA dynamics.
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用于 DNA 动力学研究的自旋标记补骨脂素探针。
DOI：
10.1021/bi00045a022
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Hearst,JE
中科院分区：
文献类型：
--
作者：
Spielmann,HP;Chi,DY;Hunt,NG;Klein,MP;Hearst,JE
1995年7月5日收到的修订版Mandarin pt ®摘要：六个氮氧自旋标记的peptiden衍生物已经合成，并作为结构和动力学研究的探针进行评估。这些衍生物的DNA寡核苷酸的序列特异性光加成导致位点特异性交联和自旋标记的寡聚体。几个双链体的电子顺磁共振（EPR）谱的观察到的一般线形特征的比较与模拟EPR谱的大小rangingin从8到46个碱基对表明，氮氧自旋标记探针报告的全球翻滚运动的低聚物。虽然没有明显的大幅度运动的peptienother比整体翻滚的DNA在调查的时间尺度上，有迹象表明弯曲和其他残余运动。（A）BC核酸内切酶DNA修复系统检测DNA的结构或动力学特征，其区分受损和未受损的DNA，并且独立于病变的内在结构。NMR研究表明，peptide encross连接的DNA改变了加合物附近的骨架动力学和构象群体[Emsley et al.等人（1993）J. Am. 115，7765-7771; Spielmann等人，Chem.Soc.115，7765-7771;等人（1995）Proc. Acad. Sci.联合SA. 92，2345-2349]，我们认为识别待修复的损伤的信号在糖-磷酸骨架中，而不是在受损的碱基中。
Revised Manuscript Received July 5, 1995® abstract: Six nitroxide spin-labeled psoralen derivatives have been synthesized and evaluated as probes for structural and dynamic studies. Sequence specific photoaddition of these derivatives to DNA oligonucleotides resulted in site-specifically cross-linked and spin-labeled oligomers. Comparison of the general line shape features of the observed electron paramagnetic resonance(EPR) spectra of several duplexes rangingin size from 8 to 46 base pairs with simulated EPR spectra indicate that the nitroxide spin-label probe reports the global tumbling motion of the oligomers. While there is no apparent large amplitude motion of the psoralenother than the overall tumbling of the DNA on the time scales investigated, there are indications of bending and other residual motions. The (A) BC excinuclease DNA repair system detects structural or dynamic features of the DNA that distinguish betweendamaged and undamaged DNA and are independent of the intrinsic structure of the lesion. NMR studies have shown that psoralencross-linked DNA has altered backbone dynamics and conformational populations in the immediate vicinity of the adduct [Emsley et al.(1993) J. Am. Chem. Soc. 115, 7765—7771; Spielmann et al.(1995) Proc. Natl. Acad. Sci. U. SA. 92, 2345—2349], We suggested that the signal for recognition of a lesion to be repaired is in the sugar—phosphate backbone and not in the damaged base (s).
补骨脂素作为核酸结构与功能探针的应用
DOI：
10.1002/chin.199116327
发表时间：
1991
期刊：
ChemInform
影响因子：
--
作者：
Y. Shi;S. Lipson;D. Y. Chi;H. Spielmann;J. Monforte;J. Hearst
通讯作者：
J. Hearst