Development of an Enzyme-Linked Immunosorbent Assay for Determination of the Miticide Bromopropylate
Development of an Enzyme-Linked Immunosorbent Assay for Determination of the Miticide Bromopropylate
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DOI：
10.1021/jf802821n
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发表时间：
2009-01-28
影响因子：
6.1
通讯作者：
Marco, M. -Pilar
中科院分区：
文献类型：
--
作者：
Ramon-Azcon, Javier;Sanchez-Baeza, Francisco;Marco, M. -Pilar
本文首次建立了杀虫剂溴丙酯（BP）的免疫分析方法。设计了免疫半抗原的化学结构，以最大限度地识别BP的双溴苯基。因此，该试验使用了针对从马蹄中偶联的2,2-二（4-溴苯基）- n -2-羟基乙酰胺-丁酸（半抗原2）的多克隆抗体。螃蟹。建立了一种异源间接竞争性酶联免疫吸附试验（ELISA），可检测血压低至0.14 μ g L-1。该分析已被证明能容忍大范围的离子强度和pH值。对该免疫分析法的选择性研究表明，该方法对结构中含有双卤代苯基的相关农药具有较高的识别能力。其他杀虫剂不干扰使用这种免疫化学技术分析BP。初步实验表明，BP可以在低至0.16 μ g L-1的白葡萄酒样品中直接分析，而无需在ELISA之前进行清理程序。
This paper reports for the first time the development of an immunoassay for the analysis of the miticide bromopropylate (BP). The chemical structure of the immunizing haptens was designed to maximize the recognition of the bis-bromophenyl group of BP. Thus, the assay uses polyclonal antibodies raised against 2,2-bis(4-bromophenyl)-N-2-hydroxyacetamide-butanoic acid (hapten 2) conjugated to keyhole limpet hemocyanin from horseshoe. crab. A heterologous indirect competitive enzyme-linked immunosorbent assay (ELISA) has been developed that can detect BP down to 0.14 mu g L-1. The assay has been proven to tolerate a wide range of ionic strengths and pH values. Studies on the selectivity of this immunoassay have demonstrated a high recognition of related pesticides that contain a bis-halophenyl group in their structure. Other pesticides do not interfere in the analysis of BP using this immunochemical technique. Preliminary experiments have shown that BP can be directly analyzed in white wine samples down to 0.16 mu g L-1 without the necessity of a cleanup procedure prior to the ELISA.