Spread of new variant RHDV in domestic rabbits on the Iberian Peninsula
Spread of new variant RHDV in domestic rabbits on the Iberian Peninsula
复制标题
DOI：
10.1016/j.vetmic.2013.12.015
复制
发表时间：
2014-02-21
影响因子：
3.3
通讯作者：
Parra, Francisco
中科院分区：
文献类型：
--
作者：
Dalton, Kevin P.;Nicieza, Ines;Parra, Francisco
最近在西班牙观察到幼兔爆发兔出血性疾病。在这项研究中，我们使用 RT-PCR 和抗原捕获 ELISA 追踪了一年多时间里从兔子养殖场采集的样本中 RHDV 变种的传播情况。对分离株进行测序，并与经典和变种 RHDV 毒株进行比较，并进行系统发育分析。在 11 天的幼崽中就观察到了死亡。超过 50% 的死亡兔子之前曾使用市售灭活疫苗接种过 RHDV 疫苗，这表明假定缺乏针对变种 RHDV 的保护。 2012 年西班牙农场所研究的绝大多数疫情 (94.5%) 都是由变异 RHDV 造成的，55 个农场中只有 3 个受到经典 RHDV 的影响。数据表明，变异型 RHDV 在相对较短的时间内已在西班牙多个省份传播，很大程度上取代了之前占主导地位的 G1 RHDV 基因型。考虑到缺乏针对 RHDV 变异株的有效疫苗，应采取严格的疾病控制和更高的警惕措施。 (C) 2013 Elsevier B.V. 保留所有权利。
Rabbit hemorrhagic disease outbreaks in young rabbits have been recently observed in Spain. In this study we have tracked the spread of variant RHDV in samples collected from rabbit farms over a period of more than one year using RT-PCR and antigen-capture ELISA. The isolates were sequenced and compared to classic and variant RHDV strains and phylogenetic analyses were conducted. Mortalities have been observed in kits as young as 11 days. More than 50% of the dead rabbits had been previously vaccinated against RHDV using commercially available inactivated vaccines indicating a putative lack of protection against the variant RHDV. The large majority of the studied outbreaks (94.5%) in Spanish farms during 2012 were due to variant RHDV and only 3 out of the 55 farms were affected by classic RHDV. The data demonstrates that the variant RHDV has spread through a large number of Spanish provinces in a relatively short period of time, largely replacing the previously predominant G1 RHDV genotypes. Considering the lack of efficient vaccines against the variant RHDV strains strict disease control and greater vigilance measures should be put in place. (C) 2013 Elsevier B.V. All rights reserved.