Development of a new model system to study microbial colonization on dentures.
Development of a new model system to study microbial colonization on dentures.
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DOI：
10.1111/jopr.12002
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发表时间：
2013-07
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影响因子：
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通讯作者：
Tingxi Wu;Wei Hu;Lihong Guo;M. Finnegan;D. Bradshaw;P. Webster;Z. Loewy;Xuedong Zhou;W. Shi;R. Lux
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文献类型：
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作者：
Tingxi Wu;Wei Hu;Lihong Guo;M. Finnegan;D. Bradshaw;P. Webster;Z. Loewy;Xuedong Zhou;W. Shi;R. Lux
义齿常被多种微生物（包括白色念珠菌）定植，这些微生物可引起义齿性口炎。以前已经建立了几种体外模型来研究义齿相关的微生物定植和评价义齿清洁剂的治疗效果;然而，这些模型通常无法理解义齿表面的复杂拓扑结构和异质性，并且缺乏准确测量微生物定植的有效方法。本研究的目的是研究微生物定植与一个新的模型系统的基础上真实的义齿，更真实地模拟体内条件。材料与方法用扫描电镜观察义齿不同部位表面的拓扑结构。使用C。以白色念珠菌为模型微生物，建立了不同义齿表面的微生物定植。此外，我们还采用了改良的MTT（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑）比色法定量C。在不需要去除生物膜的情况下观察义齿上的白色念珠菌定植，并评估义齿清洁剂的治疗效果。结果义齿不同部位表面的拓扑结构存在显著差异，其中未抛光侧的压痕和气孔数量最多。不同的义齿表面支持不同的微生物定植，未抛光的一面含有最高水平的微生物定植和生物膜形成。此外，改良的MTT比色法被证明是一种准确的测定义齿上生物膜形成和评价义齿清洁剂治疗效果的方法。结论该模型结合MTT比色法可作为研究义齿相关微生物学和治疗方法的有效工具。
PURPOSE Dentures are often colonized with a variety of microorganisms, including Candida albicans, that contribute to denture stomatitis. Several in vitro models have been previously established to study denture-related microbial colonization and evaluate treatment efficacy of denture cleansers; however, those models typically fail to appreciate the complex topology and heterogeneity of denture surfaces and lack effective ways to accurately measure microbial colonization. The purpose of this study was to study microbial colonization with a new model system based on real dentures, to more realistically mimic in vivo conditions. MATERIALS AND METHODS Scanning electron microscopy was used to observe topological structures among surfaces from different parts of the denture. Employing C. albicans as a model microorganism, we established microbial colonization on different denture surfaces. Moreover, we applied a modified MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) colorimetric assay to quantify C. albicans colonization on dentures without the necessity of biofilm removal and to evaluate treatment efficacy of denture cleansers. RESULTS There were significant variations in topological structures among surfaces from different parts of the denture, with the unpolished side having the highest amounts of indentations and pores. The distinct denture surfaces support microbial colonization differently, with the unpolished side containing the highest level of microbial colonization and biofilm formation. Furthermore, the modified MTT colorimetric assay proved to be an accurate assay to measure biofilm formation on dentures and evaluate treatment efficacy of denture cleansers. CONCLUSION This new denture model system in conjunction with the MTT colorimetric assay is a valuable tool to study denture-related microbiology and treatment approaches.