"Giant" blastoconidia of Candida albicans:: Morphologic presentation and concepts regarding their production
"Giant" blastoconidia of Candida albicans:: Morphologic presentation and concepts regarding their production
复制标题
DOI：
10.1016/s0732-8893(99)00013-9
复制
发表时间：
1999-05-01
影响因子：
2.9
通讯作者：
Abrams, J
中科院分区：
文献类型：
--
作者：
Bottone, EJ;Horga, M;Abrams, J
白色念珠菌通常产生直径2到8微米的胚芽孢子。白念珠菌分离株(命名为BH)在添加了IsoVitalex的商品巧克力琼脂上生长后，观察到明显增大的“巨型”(约30亩m)胚泡分生孢子(美国马里兰州科克斯维尔的BBL-微生物系统公司)。从形态上看，“巨型”偏向分生孢子的形态多样，如具线形折痕的胚生分生孢子，单个宽基的芽类似于皮炎芽孢杆菌，多个芽类似于巴西拟球孢子虫，或椭圆形。“巨型”胚生孢子含有一个大的椭圆形透明液泡，占胚生孢子的50%以上。从这些胚芽分生孢子发出的假菌丝也被放大，并含有类似的椭圆形包裹体。很少观察到“巨大”的胚芽孢子，胚孢子内均匀排列着贴生或内化的胚生孢子。“巨大”或扩大的胚芽孢子产量是恒定的，通常接近组成单个菌落的胚孢子单位的10%至20%，无论继代培养的数量如何。添加了Remel(Lexana，KS，USA)巧克力琼脂的IsoVitalex，但没有添加其他各种培养基，也能产生巨大的胚芽孢子。因此，II推测，IsoVitalex的一个成分(S)激活/阻止存在于白念珠菌芽胞分生孢子精选克隆中的基因，导致“巨大的”或扩大的胚芽孢子生成。(C)1999年爱思唯尔科学公司。
Candida albicans normally produces blastoconidia measuring 2 to 8 mu m in diameter. Markedly enlarged "giant" (approximate to 30 mu m) blastoconidia of a C. albicans isolate (designated BH) were observed after growth on commercially prepared chocolate agar already supplemented with IsoVitalex (BBL-Microbiology Systems, Cockeysville, MD, USA). Morphalogically, "giant" bias toconidia presented a spectrum of forms such as blastoconidia with linear creases, with a single broad-based bud resembling Blastomyces dermatididis, with multiple buds resembling Paracoccidioides brasiliensis, or elliptical in shape. "Giant" blastoconidia contained a large oval clear vacuole occupying greater than 50% of the blastoconidium. Pseudohyphae emanating from these blastoconidia were also enlarged and contained a similar oval inclusion. Rarely observed were "giant" blastoconidia with either adherent or internalized blastoconidia uniformly arranged within the blastoconidium. "Giant" or enlarged blastoconidia production was constant, usually approaching 10 to 20% of the blastoconidial units comprising a single colony, irrespective of the number of subcultures. IsoVitalex supplementation of Remel (Lexana, KS, USA) chocolate agar but not a variety of other media also resulted in "giant" blastoconidia production. II is, therefore, theorized that a component(s) of IsoVitalex activates/blocks a gene present in select clones of C. albicans blastoconidia resulting in "giant" or enlarged blastoconidiogenesis. (C) 1999 Elsevier Science Inc.