Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus -: Delineation by expression of CD27, IgD, and CD95
Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus -: Delineation by expression of CD27, IgD, and CD95
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DOI：
10.1002/art.23498
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发表时间：
2008-06-01
影响因子：
--
通讯作者：
Doerner, Thomas
中科院分区：
文献类型：
--
作者：
Jacobi, Annett M.;Reiter, Karin;Doerner, Thomas
目标。对系统性红斑狼疮（SLE）患者外周血B细胞亚群的分析提供了特异性改变的证据，如cd27++浆细胞/原细胞和移行性B细胞的扩增。然而，狼疮患者的记忆B细胞尚未被彻底研究，直到最近才在狼疮患者的外周血中发现了CD27-记忆B细胞亚群。本研究聚焦于CD27-B细胞，旨在鉴定SLE患者外周血B细胞亚群的异常。采用多参数流式细胞术和单细胞逆转录-重链转录聚合酶链反应技术，对3组独立的狼疮患者进行CD27记忆B细胞的表征。我们确定了CD27-，IgD-，CD95+记忆B细胞的均匀亚群，其活化表型在疾病发作患者中增加，并且与疾病活动性和血清学异常相关。相反，发现CD27- IgD- B细胞的整个亚群是异质的，与狼疮活动没有显著相关性，并且在细菌感染患者中也增加。我们得出结论，CD95是一种有用的标记物，可以识别具有活化表型的CD27-记忆B细胞，这可能作为狼疮活性的生物标志物，并作为进一步研究的目标，旨在阐明这些细胞的致病潜力以及CD27-，IgD-，CD95+记忆B细胞亚群的产生和调节机制。
Objective. Analysis of peripheral B cell subsets in patients with systemic lupus erythematosus (SLE) has provided evidence of specific alterations, such as an expansion of CD27++ plasma cells/blasts and transitional B cells. However, memory B cells in lupus have not been thoroughly investigated, and only recently a CD27- memory B cell subset was identified in the peripheral blood of lupus patients. Focusing on CD27-B cells, this study aimed to identify abnormalities in peripheral B cell subsets in patients with SLE.,Methods. Three independent cohorts of lupus patients were used to characterize CD27- memory B cells, using multiparameter flow cytometry and single-cell reverse transcription-polymerase chain reaction of heavy-chain transcripts.Results. We identified a homogeneous subset of CD27-,IgD-,CD95+ memory B cells with an activated phenotype that was increased in patients with disease flares and that correlated with disease activity and serologic abnormalities. In contrast, the entire subset of CD27--,IgD- B cells was found to be heterogeneous, did not correlate significantly with lupus activity, and was also increased in patients with bacterial infections.Conclusion. We conclude that CD95 is a useful marker to identify CD27- memory B cells with an activated phenotype, which might serve as a biomarker for lupus activity and as a target of further investigations aiming to elucidate the pathogenic potential of these cells and the mechanisms involved in the generation as well as regulation of this CD27-,IgD-,CD95+ memory B cell subset.