Isocratic separation of ginsenosides by high-performance liquid chromatography on a diol column at subambient temperatures
Isocratic separation of ginsenosides by high-performance liquid chromatography on a diol column at subambient temperatures
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DOI：
10.1007/s00216-006-0392-7
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发表时间：
2006-04
影响因子：
4.3
通讯作者：
Dawei Lou;Yoshihiro Saito;P. Zarzycki;M. Ogawa;K. Jinno
中科院分区：
文献类型：
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作者：
Dawei Lou;Yoshihiro Saito;P. Zarzycki;M. Ogawa;K. Jinno
建立了一种改进的高效液相色谱法分离多种黄芪皂苷的方法。通过应用二元移动的流动相（乙腈/水，82：18 v/v）和二醇柱（LiChrospher 100 Diol）研究温度（0至25°C）对皂苷的保留和分离的影响。柱温是研究组分保留和分离的重要参数之一。用van 't霍夫曲线估算了保留过程的焓变（ΔH°）和熵变（ΔS°），并讨论了可能的保留机理。以最大保留时间（tmax）、最小分离度（Rsmin）和相对分离度乘积（r）为优化指标，选择最佳分离条件。接近14°C的温度区域提供了最高的选择性，并且在色谱图上几乎相等地分布了皂苷峰。在这种等度条件下，色谱标准品和选定的人参样品在不到16分钟的时间内实现了良好的分离。
An improved high-performance liquid chromatographic method for separation of a number of ginsenosides has been developed. The influence of temperature (from 0 to 25°C) on the retention and separation of the ginsenosides was studied by applying a binary mobile phase (acetonitrile/water, 82:18 v/v) and a diol column (LiChrospher 100 Diol). The column temperature is one of the more important parameters for the retention and separation of the components investigated. Selected thermodynamic parameters, including changes of enthalpy (ΔH°) and entropy (ΔS°), were estimated from linear van’t Hoff plots, and possible retention mechanisms were discussed. Moreover, the best separation conditions were selected based on optimization criteria including maximum retention time (tR max), minimum resolution (Rsmin), and relative resolution product (r). Temperature regions close to 14°C offered the highest selectivity and almost equal distribution of the ginsenosides peaks across the chromatogram. Under such isocratic conditions, excellent separation of chromatographic standards and selected ginseng samples was achieved in less than 16 min.