A general method for improving spatial resolution by optimization of electron multiplication in CCD imaging.
A general method for improving spatial resolution by optimization of electron multiplication in CCD imaging.
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DOI：
10.1364/oe.18.005199
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发表时间：
2010-03-01
期刊：
影响因子：
3.8
通讯作者：
Tseng Y
中科院分区：
文献类型：
--
作者：
Wu PH;Nelson N;Tseng Y
电子倍增电荷耦合器件(EMCCD)相机具有电子倍增功能，可以有效地将微弱入射光子信号转换为放大的电子输出，从而大大提高采集图像的对比度。这种设备已经成为单细胞生物物理分析中流行的光子探测器，以增强亚细胞图像。然而，这种测量中的分辨率与EMCCD相机中的电子倍增设置之间的定量关系并不是很清楚。因此，我们开发了一种方法来表征在整个入射光强范围内信噪比(SNR)与EM增益设置的精确依赖关系。这些信息被进一步用来评估EMCCD在亚细胞粒子跟踪中的性能。我们得出结论，在这些实验中，存在获得最佳信噪比和最佳空间分辨率的最佳EM增益设置。如果使用不当，电子倍增会降低信噪比，增加空间误差。
The electron-multiplying charge-coupled device (EMCCD) camera possesses an electron multiplying function that can effectively convert the weak incident photon signal to amplified electron output, thereby greatly enhancing the contrast of the acquired images. This device has become a popular photon detector in single-cell biophysical assays to enhance subcellular images. However, the quantitative relationship between the resolution in such measurements and the electron multiplication setting in the EMCCD camera is not well-understood. We therefore developed a method to characterize the exact dependence of the signal-to-noise-ratio (SNR) on EM gain settings over a full range of incident light intensity. This information was further used to evaluate the EMCCD performance in subcellular particle tracking. We conclude that there are optimal EM gain settings for achieving the best SNR and the best spatial resolution in these experiments. If it is not used optimally, electron multiplication can decrease the SNR and increases spatial error.