IDENTIFICATION OF PROTEOLYTIC RUMEN BACTERIA ISOLATED FROM NEW-ZEALAND CATTLE
IDENTIFICATION OF PROTEOLYTIC RUMEN BACTERIA ISOLATED FROM NEW-ZEALAND CATTLE
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DOI：
10.1111/j.1365-2672.1995.tb03119.x
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发表时间：
1995-07-01
期刊：
影响因子：
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通讯作者：
REILLY, K
中科院分区：
文献类型：
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作者：
ATTWOOD, GT;REILLY, K
对采自新西兰放牧牛场的212株瘤胃细菌进行了蛋白酶活性测定。经形态、碳源利用、革兰氏染色、生化试验和发酵产物分析鉴定，37%的菌株的活性大于或等于瘤胃蛋白水解菌普雷沃特拉菌。系统聚类分析表明，A群有26株菌株与牛链球菌参考菌株聚在一起，C群有3株菌株与纤维分离丁状弧菌参考菌株聚在一起，B群(10株)和D群(3株)不与其余瘤胃菌型菌株聚在一起。进一步的试验确定B群的菌株为布达伊真细菌，而D群的菌株与纤维杆菌最为相似，并被描述为类纤维杆菌。未聚类菌株C21a经鉴定为瘤胃假单胞菌。这些蛋白分解细菌种群的意义被讨论与新西兰反刍动物的蛋白质分解有关。
The protease activities of 212 strains of rumen bacteria isolated from New Zealand cattle grazing pasture were measured. Thirty-seven per cent of strains had activity greater than or equal to the proteolytic rumen bacterium Prevotella ruminicola and 43 of these isolates were identified by morphology, carbon source utilization, Gram stain, biochemical tests and fermentation end-product analysis. Hierarchical Cluster Analysis showed that the strains formed four clusters: cluster A contained 26 strains and clustered with a reference strain of Streptococcus bovis; cluster C contained three strains and clustered with a reference strain of Butyrivibrio fibrisolvens, while clusters B (10 strains) and D (three strains) did not cluster with any of the remaining rumen bacterial type strains. Further tests identified strains of cluster B as Eubacterium budayi, while cluster D strains most closely resembled B. fibrisolvens and were described as B. fibrisolvens-like. An unclustered strain, C21a, was identified as P. ruminicola. The significance of these proteolytic bacterial populations is discussed in relation to protein breakdown in New Zealand ruminants.