Antibodies to CD11b, CD68, and lectin label neutrophils rather than microglia in traumatic and ischemic brain lesions
Antibodies to CD11b, CD68, and lectin label neutrophils rather than microglia in traumatic and ischemic brain lesions
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DOI：
10.1002/jnr.21198
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发表时间：
2007-04-01
影响因子：
4.2
通讯作者：
Tanaka, Junya
中科院分区：
文献类型：
--
作者：
Matsumoto, Hiroaki;Kumon, Yoshiaki;Tanaka, Junya
人们认为，驻留的静止小胶质细胞通过增殖和产生许多生物活性物质，包括促炎细胞因子和一氧化氮（NO），对大脑中的各种病理事件做出快速反应。在这项研究中，我们研究了小胶质细胞在成熟大鼠大脑中由刺伤和大脑中动脉短暂闭塞 90 分钟引起的创伤性和缺血性病变中的反应。尽管TUNEL染色显示，许多lba1(+)常驻小胶质细胞在脑损伤发生后24小时内在病变核心发生凋亡变性，但大量小、圆形、同源凝集素B4(+)/CD11b(+)/CD68(+)细胞位于病变核心。这些直径为7-9μm的小圆形细胞和多形核表达中性粒细胞特异性弹性蛋白酶、碱性磷酸酶和血小板激活因子受体。因此，它们不是激活的小胶质细胞，而是中性粒细胞。诱导型一氧化氮合成酶（iNOS）抗体的免疫组织化学染色表明，大多数iNOS（+）细胞是中性粒细胞。使用 RT-PCR 和免疫印迹的空间和动力学分析结果与免疫组织化学观察结果一致。这些结果表明有必要重新评估关于激活的小胶质细胞在严重神经病理事件中的作用的传统观点。请注意，传统的小胶质细胞标记物异凝集素 B4、CD11b 和 CD68 对于小胶质细胞并不具有特异性，特别是在病理性大脑中。 (c) 2007 年 Wiley-Liss, Inc.
Resident quiescent microglia have been thought to respond rapidly to various pathologic events in the brain by proliferating and producing many bioactive substances, including proinflammatory cytokines and nitric oxide (NO). In this study, we investigated the reaction of microglia in traumatic and ischemic lesions caused by stab wounds and the transient 90-min occlusion of middle cerebral artery in a mature rat brain. Although many lba1(+) resident microglia underwent apoptotic degeneration in the lesion core within 24 hr after the onset of the brain insult as revealed by TUNEL staining, numerous small, round, isolectin B4(+)/CD11b(+)/CD68(+) cells were localized in the lesion core. These small, round cells with diameters of 7-9 mu m and polymorph nuclei expressed neutrophil-specific elastase, alkaline phosphatase, and platelet-activating factor receptor. Accordingly, they were not activated microglia but neutrophils. Immunohistochemical staining with antibodies to inducible NO synthase (iNOS) showed that most iNOS(+) cells were neutrophils. The results from spatial and kinetic analyses using RT-PCR and immunoblotting were consistent with the immunohistochemical observations. These results suggest the necessity of reevaluating the traditional view on the roles of activated microglia in severe neuropathologic events. Note that the traditional microglial markers isolectin B4, CD11b, and CD68 are not specific for microglia, particularly in a pathologic brain. (c) 2007 Wiley-Liss, Inc.