Genetic Linkage Map Construction and QTL Analysis of Two Interspecific Reproductive Isolation Traits in Sponge Gourd.
Genetic Linkage Map Construction and QTL Analysis of Two Interspecific Reproductive Isolation Traits in Sponge Gourd.
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丝瓜两个种间生殖隔离性状遗传连锁图谱构建及QTL分析。
DOI：
10.3389/fpls.2016.00980
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发表时间：
2016
影响因子：
5.6
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Wu H;He X;Gong H;Luo S;Li M;Chen J;Zhang C;Yu T;Huang W;Luo J
以丝瓜（Luffa acutangula（L.）Roxb.和L.cylindrica（L.）罗姆具有很强的杂种优势。但是，一些生殖隔离性状阻碍了它们的正常杂交和结实，这主要是由开花时间和杂种花粉不育性造成的。为了研究两个种间生殖隔离性状的遗传基础，以S1174 [L. Acutangula（L.）Roxb.]和93075 [L. [医]圆筒草Roem.]。该图谱的总长度为1436.12 cM，标记间的平均长度为8.11 cM，由177个EST-SSR标记组成，分布在14个连锁群中，平均每个连锁群长度为102.58 cM。在遗传连锁图谱的基础上，对丝瓜开花期和杂种雄性不育性两个生殖隔离性状进行了QTL定位。在LG 1上检测到2个与开花时间相关的QTL.这两个QTL的累计贡献率分别为38.07%和15.36%，每个QTL的累计贡献率分别为22.71%。在LG 1（qPF 1.1和qPF 1.2）、LG 3（qPF 3）和LG 7（qPF 7）上检测到4个影响花粉育性的QTL。这4个QTLs对叶片光合作用的贡献率在2.91%~ 16.79%之间，共占总光合作用的39.98%。我们新开发的EST-SSR标记和连锁图谱在基因定位、比较基因组学和分子标记辅助育种等方面具有重要的应用价值。研究结果也为丝瓜种间生殖隔离相关基因的克隆和杂种优势的利用奠定了基础。
The hybrids between Luffa acutangula (L.) Roxb. and L.cylindrica (L.) Roem. have strong heterosis effects. However, some reproductive isolation traits hindered their normal hybridization and fructification, which was mainly caused by the flowering time and hybrid pollen sterility. In order to study the genetic basis of two interspecific reproductive isolation traits, we constructed a genetic linkage map using an F2 population derived from a cross between S1174 [L. acutangula (L.) Roxb.] and 93075 [L. cylindrica (L.) Roem.]. The map spans 1436.12 CentiMorgans (cM), with an average of 8.11 cM among markers, and consists of 177 EST-SSR markers distributed in 14 linkage groups (LG) with an average of 102.58 cM per LG. Meanwhile, we conducted colinearity analysis between the sequences of EST-SSR markers and the genomic sequences of cucumber, melon and watermelon. On the basis of genetic linkage map, we conducted QTL mapping of two reproductive isolation traits in sponge gourd, which were the flowering time and hybrid male sterility. Two putative QTLs associated with flowering time (FT) were both detected on LG 1. The accumulated contribution of these two QTLs explained 38.07% of the total phenotypic variance (PV), and each QTL explained 15.36 and 22.71% of the PV respectively. Four QTLs for pollen fertility (PF) were identified on LG 1 (qPF1.1 and qPF1.2), LG 3 (qPF3) and LG 7 (qPF7), respectively. The percentage of PF explained by these QTLs varied from 2.91 to 16.79%, and all together the four QTLs accounted for 39.98% of the total PV. Our newly developed EST-SSR markers and linkage map are very useful for gene mapping, comparative genomics and molecular marker-assisted breeding. These QTLs for interspecific reproductive isolation will also contribute to the cloning of genes relating to interspecific reproductive isolation and the utilization of interspecific heterosis in sponge gourd in further studies.