Facial Nerve Repair With Gore-Tex Tube and Adipose-Derived Stem Cells: An Animal Study in Dogs
Facial Nerve Repair With Gore-Tex Tube and Adipose-Derived Stem Cells: An Animal Study in Dogs
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DOI：
10.1016/j.joms.2012.05.025
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发表时间：
2013-03-01
影响因子：
1.9
通讯作者：
Zalzali, Haidar
中科院分区：
文献类型：
--
作者：
Ghoreishian, Mehdi;Rezaei, Majid;Zalzali, Haidar
目的：合成导管一直被认为是神经重建手术中的一种可行的选择。它们解决了腔内插管的目标，并消除了自体移植的缺点。然而，尽管所有的报道都很成功，但没有一篇报道包含神经修复所需的生长因子或基本细胞等再生特征。作者评价了Gore-tex管内脂肪干细胞促进面神经修复的能力。材料和方法：从7只杂种犬的自体脂肪组织中提取未分化的间充质干细胞。面神经额支被切断。用海藻酸盐水凝胶包裹的未分化脂肪干细胞填充扩张的聚四氟乙烯管修复7 mm的间隙。对照侧仅用管子和藻酸盐修复。结果：除2例对照侧外，其余均在管内形成有组织的神经组织。与正常神经直径相比，实验组和对照组神经直径分别减少29%和39%。两组神经丝阳性轴突计数均为正常值的67%。两组间的组织形态计量学参数无显著差异。神经传导速度试验组(28.5+/-3.5m/S)明显高于对照组(16.2+/-7m/S)。实验组的动作电位最大波幅(1.86+/-0.24 mV)也高于对照组(1.45+/-0.49 mV)。结论：在Gore-Tex管中加入干细胞，从功能角度促进了神经修复。然而，为了获得更好的功能和组织学结果，分化的雪旺细胞和其他介质可能是必要的。(C)2013美国口腔颌面外科医生协会口腔颌面外科杂志71：577-587,2013
Purpose: Synthetic conduits have been considered a viable option in nerve reconstructive procedures. They address the goal of entubulization and eliminate the disadvantages of autografts. However, despite all successful reports, none has contained regeneration characteristics, such as growth factors or essential cells, for nerve repair. The authors evaluated the capability of adipose-derived stem cells in Gore-Tex tubes to enhance facial nerve repair.Materials and Methods: Undifferentiated mesenchymal stem cells were extracted from the autogenous adipose tissues of 7 mongrel dogs. The frontal branch of the facial nerve was transected. A gap size of 7 mm was repaired with an expanded polytetrafluoroethylene tube filled with undifferentiated adipose-derived stem cells encapsulated in alginate hydrogel. The control sides were repaired with the tube and alginate alone. The healing phase was 12 weeks.Results: Except in 2 control sides, an organized neural tissue was formed within the tubes. Compared with the normal nerve diameter, there was a decreased ratio of 29% and 39% in the experimental and control groups, respectively. Neurofilament-positive axon counts were 67% of normal values in the 2 groups. There was no significant difference between groups in histomorphometric parameters. Nerve conduction velocity in the experimental group (28.5 +/- 3.5 m/s) was significantly greater than in the control group (16.2 +/- 7 m/s). The experimental group also exhibited a greater maximal amplitude of action potential (1.86 +/- 0.24 mV) than the control group (1.45 +/- 0.49 mV).Conclusions: Addition of stem cells in the Gore-Tex tube enhanced the neural repair from a functional standpoint. However, for better functional and histologic results, differentiated Schwann cells and other mediators may be warranted. (C) 2013 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 71:577-587, 2013