Acyltransferase-catalyzed cleavage of arachidonic acid from phospholipids and transfer to lysophosphatides in macrophages derived from bone marrow. Comparison of different donor- and acceptor substrate combinations.
Acyltransferase-catalyzed cleavage of arachidonic acid from phospholipids and transfer to lysophosphatides in macrophages derived from bone marrow. Comparison of different donor- and acceptor substrate combinations.
复制标题
酰基转移酶催化花生四烯酸从磷脂上裂解并转移到骨髓巨噬细胞中的溶血磷脂。
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
E. Ferber
中科院分区：
文献类型：
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作者：
I. Flesch;B. Ecker;E. Ferber
先前的一篇论文表明，在预标记的小鼠胸腺细胞中，发生 CoA 介导的花生四烯酸从磷脂酰胆碱到溶血磷脂酰乙醇胺的直接转移，这不涉及游离脂肪酸的中间形成。该转移不依赖于 ATP，并由酰基辅酶 A 催化：溶血磷脂酰基转移酶反向操作。在预标记的胸腺细胞中，磷脂酰胆碱是唯一的花生四烯酰供体，溶血磷脂酰乙醇胺是唯一的溶血受体。在小鼠骨髓来源的巨噬细胞中，检测到一系列 CoA 介导的转移反应，导致花生四烯酸在磷脂之间重新分布。使用外源底物，会发生从 1-酰基-2-花生四烯酰甘油磷酸胆碱到溶血磷脂酰乙醇胺的双向转移。观察到从1-酰基-2-花生四烯酰甘油磷酸肌醇到溶血磷脂酰胆碱以及从1-酰基-2-花生四烯酰甘油磷酸肌醇到溶血磷脂酰乙醇胺的单向转移。质溶性溶血受体通常比相应的酰基溶血磷脂具有更弱的受体能力。在巨噬细胞中，CoA 介导的花生四烯酰基部分的转移不依赖于 ATP 和 Mg2+，并且完全被胆酸钠抑制，表明它是由酰基辅酶 A：溶血磷脂酰基转移酶催化的。
In a previous paper it was shown that in prelabeled murine thymocytes a direct CoA-mediated transfer of arachidonic acid from phosphatidylcholine to lysophosphatidylethanolamine occurs which does not involve the intermediate formation of free fatty acid. The transfer is ATP-independent and is catalyzed by the acyl-CoA: lysophosphatide acyltransferase operating in reverse. In prelabeled thymocytes phosphatidylcholine was the only arachidonoyl donor and lysophosphatidylethanolamine the only lysoacceptor. In murine bone-marrow-derived macrophages a series of CoA-mediated transfer reactions were detected leading to a redistribution of arachidonic acid between phospholipids. Using exogenous substrates a bidirectional transfer from 1-acyl-2-arachidonoylglycerophosphocholine to lysophosphatidylethanolamine occurs. An unidirectional transfer from 1-acyl-2-arachidonoylglycerophosphoinositol to lysophosphatidylcholine and from 1-acyl-2-arachidonoylglycerophosphoinositol to lysophosphatidylethanolamine was observed. Plasmalogenic lysoacceptors generally have a weaker acceptor capacity than the correspondent acyllysophospholipid. In macrophages the CoA-mediated transfer of arachidonoyl moieties is independent of ATP and Mg2+ and is totally inhibited by sodium cholate, indicating that it is catalyzed by the acyl-CoA: lysophosphatide acyltransferase.
受刺激的人血小板中花生四烯酸的释放和磷脂酸的周转。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Neufeld,EJ;Majerus,PW
通讯作者：
Majerus,PW