Genetic mosaic techniques for studying Drosophila development
Genetic mosaic techniques for studying Drosophila development
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DOI：
10.1242/dev.00774
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发表时间：
2003-11-01
期刊：
影响因子：
4.6
通讯作者：
Blair, SS
中科院分区：
文献类型：
--
作者：
Blair, SS
隐性突变的遗传筛查继续为果蝇发育遗传学的现代工作提供基础。然而，在这些筛选中分离的许多突变导致胚胎或早期幼虫致死。然而，研究这种突变对后来发育事件的影响仍然是可能的，使用遗传镶嵌技术，该技术将遗传功能的丧失或获得限制在特定的组织和细胞以及选定的发育阶段。已经开发了各种遗传镶嵌技术，这些技术导致了对苍蝇发育过程的关键见解。这些技术的变化也可用于筛选参与非胚胎模式和生长的新基因。
Genetic screens for recessive mutations continue to provide the basis for much of the modern work on Drosophila developmental genetics. However, many of the mutations isolated in these screens cause embryonic or early larval lethality. Studying the effects of such mutations on later developmental events is still possible, however, using genetic mosaic techniques, which limit losses or gains of genetic function to specific tissues and cells, and to selected stages of development. A variety of genetic mosaic techniques have been developed, and these have led to key insights into developmental processes in the fly. Variations on these techniques can also be used to screen for novel genes that are involved in non-embryonic patterning and growth.