Characterization of low temperature-induced plasma membrane lipidome remodeling combined with gene expression analysis reveals mechanisms that regulate membrane lipid desaturation in Carica papaya
Characterization of low temperature-induced plasma membrane lipidome remodeling combined with gene expression analysis reveals mechanisms that regulate membrane lipid desaturation in Carica papaya
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DOI：
10.1016/j.scienta.2020.109505
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发表时间：
2020-10-15
影响因子：
4.3
通讯作者：
Zeng, Wei
中科院分区：
文献类型：
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作者：
Chen, Mingjie;Liu, Ruiming;Zeng, Wei
番木瓜是一种冷敏感型园艺作物，其冷敏感性的机理尚不清楚，限制了其耐冷性的遗传改良。本研究采用葡聚糖-聚乙二醇两相分配系统对28 ℃和10 ℃下生长的番木瓜叶片质膜进行分离纯化，利用脂质组学方法分析质膜总脂含量，同时采用RT-qPCR方法对番木瓜叶片中脂肪酸去饱和酶的转录水平进行定量分析。我们发现，糖苷类是主要的质膜脂质种类，其次是磷脂和游离甾醇。低温处理增加了单不饱和和双不饱和磷脂含量，降低了双饱和磷脂含量，但对总磷脂含量没有影响。冷胁迫增强了CpFAD 4、CpFAD 6和CpFAB 2 - 2的转录。通路分析表明，低温诱导CpFAB 2 - 2转录和CpFAD 2酶活性在低温胁迫下质膜脂质体重构中起重要作用。我们的研究结果揭示了潜在的目标，以提高木瓜耐冷性。
Carica papaya is a cold-sensitive horticultural crop, the mechanisms underlying its cold sensitivity remains unclear which limit its genetic improvements in cold tolerance. In this study, aqueous dextran-polyethylene glycol two-phase partition systems were applied to purify plasma membrane from papaya leaves grown under 28 degrees C and 10 degrees C, the plasma membrane total lipids were analyzed by lipidomic approach; meanwhile, the transcription levels of putative fatty acid desaturases were quantified by RT-qPCR. We found that cerebrosides are dominate plasma membrane lipid species, followed by phospholipids and free sterols. Cold treatment increased the mono-unsaturated and di-unsaturated phospholipids, and decreased di-saturated phospholipids, the proportion of total phospholipids was not affected. Cold stress enhanced the transcription of CpFAD4, CpFAD6 and CpFAB2 - 2. Pathway analysis suggested that the cold induction of CpFAB2 - 2 transcription and CpFAD2 enzyme activities play important roles for plasma membrane lipidome remodeling under cold stress. Our findings uncovered potential targets to improve papaya cold tolerance.