PORCINE DIACYLGLYCEROL KINASE SEQUENCE HAS ZINC FINGER AND E-F HAND MOTIFS
PORCINE DIACYLGLYCEROL KINASE SEQUENCE HAS ZINC FINGER AND E-F HAND MOTIFS
复制标题
DOI：
10.1038/344345a0
复制
发表时间：
1990-03-22
期刊：
影响因子：
64.8
通讯作者：
TANABE, T
中科院分区：
文献类型：
--
作者：
SAKANE, F;YAMADA, K;TANABE, T
细胞刺激导致二酰甘油激酶（DGK）将第二信使二酰甘油转化为磷脂酸，从而启动磷脂酰肌醇的再合成并减弱蛋白激酶C活性1。在迄今为止发现的DGK亚型中2 -4，只有来自淋巴细胞的猪DGK 5已被详细表征3，5-7。在这里，我们报告了互补DNA克隆的分离和测序，这些克隆共同覆盖了编码猪DGK（相对分子质量80，000（80 K））的整个区域。推导出的一级结构，这个DGK包含推定的ATP结合位点，两个富含半胱氨酸的锌指样序列类似于蛋白激酶C8中发现的那些，和两个E-F手图案，典型的钙离子结合蛋白，如钙调素9。事实上，我们发现，这种DGK亚型的活性增强微摩尔浓度的Ca 2+在脱氧胆酸盐或鞘氨醇的存在下。80 K DGK的这些性质表明，其作用可能与第二信使二酰基甘油10和肌醇1，4，5-三磷酸11有关。
CELL stimulation causes diacylglycerol kinase (DGK) to convert the second messenger diacylglycerol into phosphatidate, thus initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity1. Of the DGK isoforms so far reported2–4, only porcine DGK from lymphocytes5has been characterized in detail3,5–7. Here we report the isolation and sequencing of complementary DNA clones that together cover the entire region encoding porcine DGK (relative molecular mass 80,000 (80K)). The deduced primary structure of this DGK contains the putative ATP-binding sites, two cysteine-rich zinc finger-like sequences similar to those found in protein kinase C8, and two E–F hand motifs, typical of Ca2+-binding proteins like calmodulin9. Indeed, we find that the activity of this DGK isoform is enhanced by micromolar concentrations of Ca2+in the presence of deoxycholate or sphingosine. These properties of 80K DGK indicate that its action is probably linked with both of the second messengers diacylglycerol10and inositol 1,4,5-trisphosphate11.