Learning strategy selection in the water maze and hippocampal CREB phosphorylation differ in two inbred strains of mice.
Learning strategy selection in the water maze and hippocampal CREB phosphorylation differ in two inbred strains of mice.
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两种近交系小鼠的水迷宫学习策略选择和海马 CREB 磷酸化有所不同。
DOI：
10.1101/lm.783108
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Han,Jung-Soo
中科院分区：
文献类型：
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作者：
Sung,Jin-Young;Goo,June-Seo;Lee,Dong-Eun;Jin,Da-Qing;Bizon,JenniferL;Gallagher,Michela;Han,Jung-Soo
在两种不同的近交系小鼠 C57BL/6 和 DBA/2 中评估了学习策略的选择，这两种小鼠用于开发转基因小鼠模型。雄性小鼠在水迷宫中接受了使用可见和隐藏平台试验交替块的训练方案，在此期间小鼠逃到一个位置。训练后，小鼠需要在训练期间平台所在的空间位置（位置策略）和新位置中呈现的可见平台（提示/响应策略）之间进行选择。两种品系的小鼠在训练期间的可见和隐藏平台试验中都具有相似的逃脱表现。然而，在策略偏好测试中，C57BL/6 小鼠选择位置策略的频率明显高于 DBA/2 小鼠。由于许多证据表明海马体和纹状体分别是空间/位置和提示/反应学习的重要神经基质，因此在位置或提示训练后，通过测定这两种小鼠品系中 cAMP 反应元件结合蛋白 (CREB) 和磷酸化 CREB (pCREB) 的水平来评估海马体的参与情况。结果显示，与提示训练相比，两种品系小鼠的海马 CREB 水平在放置后均显着增加。然而，海马 pCREB 水平与训练的关系是应变依赖性的；地点训练后，C57BL/6 小鼠的 pCREB 显着高于 DBA/2 小鼠，而提示训练后，品系之间的海马 pCREB 水平没有差异。这些发现表明，与地点/空间训练特别相关的 pCREB 与 C57BL/6 和 DBA/2 小鼠之间空间/地点策略偏好的差异密切相关。
Learning strategy selection was assessed in two different inbred strains of mice, C57BL/6 and DBA/2, which are used for developing genetically modified mouse models. Male mice received a training protocol in a water maze using alternating blocks of visible and hidden platform trials, during which mice escaped to a single location. After training, mice were required to choose between the spatial location where the platform had been during training (a place strategy) and a visible platform presented in a new location (a cued/response strategy). Both strains of mice had similar escape performance on the visible and hidden platform trials during training. However, in the strategy preference test, C57BL/6 mice selected a place strategy significantly more often than DBA/2 mice. Because much evidence implicates the hippocampus and striatum as important neural substrates for spatial/place and cued/response learning, respectively, the engagement of the hippocampus was then assessed after either place or cue training by determining levels of cAMP response element-binding protein (CREB) and phosphorylated CREB (pCREB) in these two mouse strains. Results revealed that hippocampal CREB levels in both strains of mice were significantly increased after place in comparison to cued training. However, the relation of hippocampal pCREB levels to training was strain dependent; pCREB was significantly higher in C57BL/6 mice than in DBA/2 mice after place training, while hippocampal pCREB levels did not differ between strains after cued training. These findings indicate that pCREB, specifically associated with place/spatial training, is closely tied to differences in spatial/place strategy preference between C57BL/6 and DBA/2 mice.