SPASMOLYTIC POLYPEPTIDE - A TREFOIL PEPTIDE SECRETED BY RAT GASTRIC MUCOUS CELLS
SPASMOLYTIC POLYPEPTIDE - A TREFOIL PEPTIDE SECRETED BY RAT GASTRIC MUCOUS CELLS
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DOI：
10.1016/0016-5085(94)90590-8
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发表时间：
1994-02-01
期刊：
影响因子：
29.4
通讯作者：
BRAND, SJ
中科院分区：
文献类型：
--
作者：
JEFFREY, GP;OATES, PS;BRAND, SJ
背景/目的：痉挛多肽（SP）是一种在消化道表达的三叶肽。本研究的目的是确定SP在大鼠胃肠道中表达的结构和分布。应用北方印迹和原位杂交技术对成年和胎鼠消化道SP基因表达进行定位。结果：SP信使RNA主要在胃中表达，在胃窦中表达最高。在胃泌素和生长抑素表达之前，胎儿胃中SP mRNA表达水平较高。令人惊讶的是，SP mRNA和肽没有共定位在胃粘膜中，SP mRNA是浅表的SP肽免疫反应在整个胃粘膜。结论：大鼠胃窦粘膜细胞分泌的P物质是一种主要由胃窦粘膜细胞分泌的肽类物质。粘附胃粘液层中高浓度的SP（约10 μmol/L）提示SP是一种结构肽而不是调节肽。
Background/Aims:Spasmolytic polypeptide (SP) is a trefoil peptide expressed in the digestive tract. This study aimed to determine the structure and distribution of SP expression in the rat gastrointestinal tract.Methods:The structure of rat SP was determined from the sequence of complementary DNAs isolated from antral RNA. SP gene expression was localized by Northern blotting and in situ hybridization in the adult and fetal rat digestive tract. Expression of the SP peptide was localized by immunocytochemistry and Western blot analysis.Results:SP messenger (m)RNA was found predominantly in the stomach with highest expression in the antrum. High levels of SP mRNA were expressed in the fetal stomach before gastrin and somatostatin expression. Surprisingly, SP mRNA and peptide did not colocalize in the gastric mucosa, SP mRNA being superficial to SP peptide immunoreactivity throughout the gastric mucosa. Abundant SP immunoreactivity was seen in the lumen of the gastric glands and the mucus layer adherent to the gastric mucosa, indicating luminal secretion.Conclusions:In the rat, SP is a peptide secreted predominantly from antral mucous cell. The high concentrations of SP in the adherent gastric mucus layer (approximately 10 μmol/L) suggest that SP functions as a structural peptide rather than a regulatory peptide.