Sperm accessory microtubules suggest the placement of Diplura as the sister-group of Insecta s.s.
Sperm accessory microtubules suggest the placement of Diplura as the sister-group of Insecta s.s.
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DOI：
10.1016/j.asd.2010.08.001
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Frati, Francesco
中科院分区：
文献类型：
--
作者：
Dallai, Romano;Mercati, David;Frati, Francesco
日本血吸虫科(JAPYX solifugus、Metaja yx braueri和OcCasja yx Japan onicus)和弯曲蜂科(Campodea sp.)精子超微结构及精子发生，以期为重建基本六足类的系统发育关系寻找潜在的特征。其精子轴丝模式为9+9+2，具有9根附生微管。然而，这些微管在形成后失去了9+2周围的通常位置，并在两个线粒体之间迁移。在蝶科动物中，有4个微管非常短，经阴性染色和连续切片后，可在细胞核下方观察到它们。附生微管管壁有13根原丝。Diplura的精子形态与其余Entognatha(原尾目+弹尾目)的精子形态非常不同。根据目前的结果，附生微管的存在表明Diplura是昆虫门的姊妹群。此外，龟科和龟科在精子成分的相对位置上有所不同，前者的轴丝始于核下方(其上方有短的顶体)，而后者具有长的顶体和与轴丝的近端平行的核。本研究还允许重新描述日本蜘蛛的雄性生殖系统。(C)2010年由爱思唯尔有限公司出版。
Sperm ultrastructure and spermiogenesis of the dipluran Japygidae (Japyx solifugus, Metajapyx braueri and Occasjapyx japonicus) and Campodeidae (Campodea sp.) were studied with the aim of looking for potential characters for the reconstruction of the phylogenetic relationships of basal hexapods. Both Japygidae and Campodeidae share a common sperm axonemal model 9 + 9 + 2, provided with nine accessory microtubules. These microtubules, however, after their formation lose the usual position around the 9 + 2 and migrate between the two mitochondria. In Japygidae, four of these microtubules are very short and were observed beneath the nucleus after negative staining and serial sections. Accessory microtubules have 13 protofilaments in their tubular wall. Diplura have a sperm morphology which is very different from that of the remaining Entognatha (Protura + Collembola). On the basis of the present results, the presence of accessory microtubules suggests that Diplura are the sister-group of the Insecta s.s.. Moreover, Japygidae and Campodeidae differ with regards to the relative position of the sperm components, the former having the axoneme starting from beneath the nucleus (above which sits the short acrosome), while the latter having a long apical acrosome and a nucleus running parallel with the proximal part of the axoneme. The present study also allowed to redescribe the male genital system of Japyx. (C) 2010 Published by Elsevier Ltd.