Identification of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible genes in human amniotic epithelial cells
Identification of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible genes in human amniotic epithelial cells
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DOI：
10.1186/1477-7827-4-27
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发表时间：
2006-05-17
影响因子：
4.4
通讯作者：
Miyamoto, Kaoru
中科院分区：
文献类型：
--
作者：
Abe, Yumiko;Sinozaki, Hiromitsu;Miyamoto, Kaoru
背景：暴露于二恶英会导致人类胎儿广泛的病理生理障碍。为了研究二恶英对胎胎盘组织的影响，我们分析了2,3,7,8-四氯二苯并对二恶英（TCDD）处理的人羊膜上皮细胞原代培养物的基因表达。方法：利用羊膜胰蛋白酶分散人羊膜上皮细胞，在添加10%胎牛血清的DME/Ham’s F12培养基中培养。镀后2周，用50 nM TCDD或DMSO（对照）处理细胞，孵育48小时，采用DNA微阵列技术和实时荧光定量PCR分析基因表达。结果：共鉴定到细胞色素p4501a1、细胞色素p4501b1等38个tcdd诱导基因。其中一个显著的基因表达谱是干扰素诱导基因的显著上调。参与干扰素基因表达和干扰素信号通路的基因也上调。此外，TCDD还增强了胶原合成或降解相关基因的表达。结论：利用DNA芯片技术和实时荧光定量PCR技术，我们在人羊膜上皮细胞中鉴定出了tcdd诱导基因，包括干扰素诱导基因和胶原合成或降解相关基因。
Background: Exposure to dioxins results in a broad range of pathophysiological disorders in human fetuses. In order to evaluate the effects of dioxins on the feto-placental tissues, we analyzed the gene expression in 2,3,7,8-tetrachlorodibenzo-p-dioxin ( TCDD) treated primary cultures of human amniotic epithelial cells.Methods: Human amniotic epithelial cells were dispersed by trypsin from amniotic membranes and cultured in DME/Ham's F12 medium supplemented with 10% FBS. Two weeks after plating, cells were treated with 50 nM TCDD or DMSO ( control), further incubated for 48 hrs, and the gene expression was analyzed by DNA microarray technology and quantitative real-time PCR.Results: Thirty eight TCDD-inducible genes, including cytochromeP4501A1 and cytochromeP4501B1, were identified. One of the remarkable profiles of the gene expression was the prominent up-regulation of interferon-inducible genes. The genes involved in the interferon gene expression and interferon signaling pathways were also up-regulated. Furthermore, the expression of genes related to collagen synthesis or degradation was enhanced by TCDD.Conclusion: Using DNA microarray and quantitative real-time PCR analyses, we identified TCDD-inducible genes, including interferon-inducible genes and genes related to collagen synthesis or degradation, in human amniotic epithelial cells.