DUAL INFECTION WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 OF DISTINCT ENVELOPE SUBTYPES IN HUMANS
DUAL INFECTION WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 OF DISTINCT ENVELOPE SUBTYPES IN HUMANS
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DOI：
10.1093/infdis/171.4.805
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发表时间：
1995-04-01
影响因子：
6.4
通讯作者：
MCCUTCHAN, FE
中科院分区：
文献类型：
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作者：
ARTENSTEIN, AW;VANCOTT, TC;MCCUTCHAN, FE
人类免疫缺陷病毒1型（HIV-1）的多个遗传亚型已在国际收集的分离株中被确定。HIV-1在泰国的流行主要是由于B和E亚型病毒。不同HIV-1亚型的双重感染表明，由一种亚型引起的抗病毒免疫可能对另一种亚型不完全具有保护作用。聚合酶链反应分型和血清学分型用于筛选来自泰国hiv -1感染受试者的一组标本。两个人同时携带env亚型B和E的HIV-1，并通过亚型特异性探针的集落杂交和来自多个分子克隆的gp120的630 bp片段的核苷酸序列分析证实了这一点。此外，这两种亚型在共培养的单个外周血单核细胞中被鉴定出来。这些数据提供了人类双重HIV-1感染的第一个证据，并加强了对多价疫苗的需求。
Multiple genetic subtypes of human immunodeficiency virus type 1 (HIV-1) have been identified among internationally collected isolates. The HIV-1 epidemic in Thailand is largely due to B and E subtypes of virus. Dual infection with distinct HIV-1 subtypes would suggest that antiviral immunity evoked by one subtype can be incompletely protective against a second. Polymerase chain reaction typing and serologic typing were used to screen a panel of specimens from HIV-1-infected subjects in Thailand. Two persons simultaneously harbored HIV-1 of env subtypes B and E, and this was confirmed by colony hybridization with subtype-specific probes and nucleotide sequence analysis of a 630-bp fragment of gp120 from multiple molecular clones. In addition, both subtypes were identified in cocultured peripheral blood mononuclear cells from 1 individual. These data provide the first evidence of dual HIV-1 infection in humans and reinforce the need for polyvalent vaccines.