Effects of hypoxia and reoxygenation on lung glutathione system.
Effects of hypoxia and reoxygenation on lung glutathione system.
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缺氧和复氧对肺谷胱甘肽系统的影响。
DOI：
10.1152/ajpheart.1990.259.2.h518
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Veal,CF
中科院分区：
文献类型：
--
作者：
Jackson,RM;Veal,CF
再扩张肺水肿（RPE）与其他器官中的再灌注（再氧合）损伤平行，因为缺氧和低灌注的肺组织在再扩张后发展增加的血管通透性和中性粒细胞浸润。本研究调查了缺氧（肺萎陷）和复氧（肺复张）后肺细胞谷胱甘肽系统。研究了两个单独的家兔组，以确定肺缺氧-复氧对1）肺谷胱甘肽过氧化物酶和还原酶活性以及2）肺组织、血浆和肺泡灌洗液总量（还原型谷胱甘肽+谷胱甘肽二硫化物）和氧化型谷胱甘肽的影响。肺萎陷3-7天或萎陷7天后2小时的复张均不影响谷胱甘肽过氧化物酶[对照组，0.36 +/- 0.04（左），0.38 +/- 0.03 U/mg DNA（右）]或还原酶[对照组，0.12 +/- 0.01（左），0.14 +/- 0.01 U/mg DNA（右）]活性。右肺复张后，右肺泡灌洗液中谷胱甘肽二硫化物浓度显著升高，而血浆中谷胱甘肽二硫化物浓度无明显变化。塌陷7天后，右肺总谷胱甘肽显著降低（-19%）。右肺复张后，左肺（-65%）和右肺（-68%）总谷胱甘肽均显著降低。在右肺再扩张后，左肺（占总谷胱甘肽的15.5 +/- 4.0%）和右肺（占总谷胱甘肽的18.7 +/- 6.3%）中氧化型谷胱甘肽的百分比均显著增加。这些数据表明，肺组织缺氧，所产生的单侧肺萎陷，与单方面减少肺总谷胱甘肽含量。右肺复氧，由于快速再扩张，造成双侧肺总谷胱甘肽含量下降，右肺和肺泡灌洗液谷胱甘肽二硫化物浓度增加。
Reexpansion pulmonary edema (RPE) parallels reperfusion (reoxygenation) injuries in other organs in that hypoxic and hypoperfused lung tissue develops increased vascular permeability and neutrophil infiltration after reexpansion. This study investigated the lung cellular glutathione system during hypoxia (produced by lung collapse) and after reoxygenation (produced by reexpansion). Two separate groups of rabbits were studied to determine effects of lung hypoxia-reoxygenation on 1) lung glutathione peroxidase and reductase enzyme activities and 2) lung tissue, plasma, and alveolar lavage fluid total (reduced glutathione plus glutathione disulfide) and oxidized glutathione. Neither lung collapse for 3-7 days nor reexpansion for 2 h after 7 days of collapse affected glutathione peroxidase [controls, 0.36 +/- 0.04 (left), 0.38 +/- 0.03 U/mg DNA (right)] or reductase [controls, 0.12 +/- 0.01 (left), 0.14 +/- 0.01 U/mg DNA (right)] activities. The concentration of glutathione disulfide increased markedly in right alveolar lavage fluid, but not in plasma, after right lung reexpansion. Right lung total glutathione decreased significantly (-19%) after 7 days of collapse. After right lung reexpansion, both left (-65%) and right (-68%) lung total glutathione decreased significantly. The percent of total glutathione present in the oxidized form increased significantly in both left (to 15.5 +/- 4.0% of total) and right (to 18.7 +/- 6.3% of total) lungs after reexpansion of the right lung. These data indicate that lung tissue hypoxia, produced by unilateral lung collapse, was associated with a unilateral decrease in lung total glutathione content. Right lung reoxygenation, due to rapid reexpansion, caused a bilateral decrease in lung total glutathione content and an increase in right lung and alveolar lavage fluid glutathione disulfide concentration.