Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China.
Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China.
复制标题
通过 MiSeq 测序对中国胰腺癌患者进行肠道微生物谱分析
DOI：
10.18632/oncotarget.18820
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发表时间：
2017-11-10
期刊：
影响因子：
--
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Ren Z;Jiang J;Xie H;Li A;Lu H;Xu S;Zhou L;Zhang H;Cui G;Chen X;Liu Y;Wu L;Qin N;Sun R;Wang W;Li L;Wang W;Zheng S
胰腺癌（PC）是一种致命的癌症。肠道菌群与PC的一些危险因素有关，如肥胖和II型糖尿病。然而，中国临床PC患者的具体肠道微生物特征尚未见报道。本前瞻性研究收集了85名PC和57名匹配的健康对照（HC），通过MiSeq测序分析微生物特征。结果表明，PC具有独特的肠道微生物特征，其肠道微生物多样性降低的部分原因是α多样性的降低。PC中的微生物变化表现为某些病原体和产脂多糖菌的增加，益生菌和产丁酸菌的减少。将梗阻病例的微生物群落与未梗阻病例进行分离。胆汁与链球菌有关。此外，在PC中，亮氨酸和LPS生物合成等23种微生物功能增强，而13种功能减少。重要的是，基于与PC相关的40个属，微生物标记获得了较高的分类能力，AUC为0.842。总之，肠道微生物谱在PC中是独特的，为非侵入性PC诊断提供了微生物标志物。
Pancreatic carcinoma (PC) is a lethal cancer. Gut microbiota is associated with some risk factors of PC, e.g. obesity and types II diabetes. However, the specific gut microbial profile in clinical PC in China has never been reported. This prospective study collected 85 PC and 57 matched healthy controls (HC) to analyze microbial characteristics by MiSeq sequencing. The results showed that gut microbial diversity was decreased in PC with an unique microbial profile, which partly attributed to its decrease of alpha diversity. Microbial alterations in PC featured by the increase of certain pathogens and lipopolysaccharides-producing bacteria, and the decrease of probiotics and butyrate-producing bacteria. Microbial community in obstruction cases was separated from the un-obstructed cases. Streptococcus was associated with the bile. Furthermore, 23 microbial functions e.g. Leucine and LPS biosynthesis were enriched, while 13 functions were reduced in PC. Importantly, based on 40 genera associated with PC, microbial markers achieves a high classification power with AUC of 0.842. In conclusion, gut microbial profile was unique in PC, providing a microbial marker for non-invasive PC diagnosis.
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影响因子：
15.8
作者：
Faca VM;Song KS;Wang H;Zhang Q;Krasnoselsky AL;Newcomb LF;Plentz RR;Gurumurthy S;Redston MS;Pitteri SJ;Pereira-Faca SR;Ireton RC;Katayama H;Glukhova V;Phanstiel D;Brenner DE;Anderson MA;Misek D;Scholler N;Urban ND;Barnett MJ;Edelstein C;Goodman GE;Thornquist MD;McIntosh MW;DePinho RA;Bardeesy N;Hanash SM
通讯作者：
Hanash SM
影响因子：
25.7
作者：
Darnaud, Marion;Faivre, Jamila;Moniaux, Nicolas
通讯作者：
Moniaux, Nicolas
DOI：
10.1097/ppo.0000000000000046
发表时间：
2014-05
期刊：
Cancer journal (Sudbury, Mass.)
影响因子：
--
作者：
Michaud DS;Izard J
通讯作者：
Izard J
影响因子：
50.3
作者：
Dapito DH;Mencin A;Gwak GY;Pradere JP;Jang MK;Mederacke I;Caviglia JM;Khiabanian H;Adeyemi A;Bataller R;Lefkowitch JH;Bower M;Friedman R;Sartor RB;Rabadan R;Schwabe RF
通讯作者：
Schwabe RF
DOI：
10.1073/pnas.1116053109
发表时间：
2012-01-10
影响因子：
11.1
作者：
Greenblum, Sharon;Turnbaugh, Peter J.;Borenstein, Elhanan
通讯作者：
Borenstein, Elhanan