The cutthroat trout Y chromosome is conserved with that of rainbow trout
The cutthroat trout Y chromosome is conserved with that of rainbow trout
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DOI：
10.1159/000138893
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发表时间：
2008-01-01
影响因子：
1.7
通讯作者：
Thorgaard, G. H.
中科院分区：
文献类型：
--
作者：
Alfaqih, M. A.;Phillips, R. B.;Thorgaard, G. H.
对虹鳟（Oncorhynchus mykiss）性染色体上先前发现的5个遗传标记进行了测试，以确定它们与黄石切喉鳟鱼（Oncorhynchus clarki bouvieri）的性位点在虹鳟与切喉鳟鱼（Oncorhynchus clarki bouvieri）的遗传杂交中的连锁关系。结果表明，虹鳟和喉鳟的性位点位于同一同源连锁群上。采用荧光原位杂交（FISH）技术，利用定位于黄石切喉鳟鱼性别位点附近的微卫星标记Omm1665，对杂交的切喉鳟鱼Y染色体进行了鉴定。切喉鳟鱼的Y染色体是亚远心的，并且在一些雄性虹鳟鱼的Y染色体短臂上缺少DAPI带。版权所有(c) 2008 S. Karger AG，巴塞尔。
Five genetic markers previously shown to be located on the sex chromosomes of rainbow trout (Oncorhynchus mykiss) were tested for linkage with the sex locus of Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri) in a genetic cross created from a rainbow x cutthroat male hybrid. We show that the sex locus of both rainbow and cutthroat trout is on the same homologous linkage group. Fluorescence in situ hybridization (FISH) using a probe for the microsatellite marker Omm1665, which maps close to the sex locus of Yellowstone cutthroat trout, was used to identify the Y chromosome of cutthroat trout in the hybrid. The Y chromosome of cutthroat trout is sub-telocentric and lacks a DAPI band found on the short arm of the Y chromosome of some rainbow trout males. Copyright (c) 2008 S. Karger AG, Basel.