Nuclear factors in B lymphoma enhance splicing of mouse membrane-bound mu mRNA in Xenopus oocytes.
Nuclear factors in B lymphoma enhance splicing of mouse membrane-bound mu mRNA in Xenopus oocytes.
复制标题
B 淋巴瘤中的核因子增强了非洲爪蟾卵母细胞中小鼠膜结合 mu mRNA 的剪接。
DOI：
10.1126/science.3124268
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Korn,LJ
中科院分区：
文献类型：
--
作者：
Tsurushita,N;Ho,L;Korn,LJ
Regulation of the synthesis of membrane-bound and secreted immunoglobulin μ heavy chains at the level of RNA processing is an important element for B cell development. The precursor μ RNA is either polyadenylated at the upstream poly(A) site (for the secreted form) or spliced (for the membrane-bound form) in a mutually exclusive manner. When the mouse μ gene linked to the SV40/HSV-TK hybrid promoter was microinjected intoXenopusoocytes, the μ messenger RNA (mRNA) was processed primarily to the secreted form. The processing pattern of μ mRNA was altered by coinjection of nuclei of mouse surface IgM-bearing B-lymphoma cells to include the synthesis of the membrane-bound form. An increase in the membrane-bound form was not observed when nuclei of IgM-secreting hybridoma cells or fibroblast cells were coinjected. Deletion of the upstream poly(A) site did not eliminate the effect of B-lymphoma nuclei suggesting that membrane-specific splicing is stimulated. Further, splicing of other μ gene introns was not affected by coinjection of B-lymphoma nuclei. These results suggest that mature B cells contain one or more transacting nuclear factors that stimulate splicing specific for membrane-bound μ mRNA.
