Sperm viability and gene expression in honey bee queens (Apis mellifera) following exposure to the neonicotinoid insecticide imidacloprid and the organophosphate acaricide coumaphos
Sperm viability and gene expression in honey bee queens (Apis mellifera) following exposure to the neonicotinoid insecticide imidacloprid and the organophosphate acaricide coumaphos
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DOI：
10.1016/j.jinsphys.2016.03.004
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发表时间：
2016-06-01
影响因子：
2.2
通讯作者：
Pettis, Jeffery S.
中科院分区：
文献类型：
--
作者：
Chaimanee, Veeranan;Evans, Jay D.;Pettis, Jeffery S.
蜜蜂数量下降是全球关注的问题。许多因素似乎导致了这些下降，包括寄生虫、病原体、营养不良和杀虫剂。在蜂蜡、花粉和梳子样品中检测到有机磷酸酯类杀螨剂coumaphos和新烟碱类杀虫剂吡虫啉的残留，这两种杀虫剂分别被广泛用于防治瓦螨和农业作物保护。在这里，我们评估了不同剂量的这些化合物对储存在蜂王精子囊中的精子活力的影响。我们的研究结果表明，亚致死剂量的吡虫啉（0.02 ppm）在治疗后7天使精子活力降低了50%。高剂量香豆福（100ppm）处理后，蜂王的精子活力呈下降趋势（约33%），但差异不显著。通过qPCR分析，测定了接触化学物质的蜂王和工蜂的发育、免疫反应和解毒相关基因的表达。数据显示，特定基因的表达水平在治疗后1天被触发。低剂量康马福（5 ppm）和吡虫啉（0.02 ppm）处理后蜂王P450亚家族基因CYP306A1、CYP4G11和CYP6AS14的表达水平降低。此外，这两种化合物在第1天抑制了蜂王抗氧化、免疫和发育相关基因的表达。在第1天观察到这些化合物在工蜂体内抗氧化剂的上调。Coumaphos也引起工人体内CYP306A1和CYP4G11的抑制。抗氧化剂似乎可以防止化学物质对蜜蜂的伤害。我们还发现，使用吡虫啉的工人DWV复制增加。这项研究清楚地表明，接触化学物质会影响蜂王精子的生存能力。(C) 2016 Elsevier Ltd.版权所有。
Honey bee population declines are of global concern. Numerous factors appear to cause these declines including parasites, pathogens, malnutrition and pesticides. Residues of the organophosphate acaricide coumaphos and the neonicotinoid insecticide imidacloprid, widely used to combat Varroa mites and for crop protection in agriculture, respectively, have been detected in wax, pollen and comb samples. Here, we assess the effects of these compounds at different doses on the viability of sperm stored in the honey bee queens' spermatheca. Our results demonstrate that sub-lethal doses of imidacloprid (0.02 ppm) decreased sperm viability by 50%, 7 days after treatment. Sperm viability was a downward trend (about 33%) in queens treated with high doses of coumaphos (100 ppm), but there was not significant difference. The expression of genes that are involved in development, immune responses and detoxification in honey bee queens and workers exposed to chemicals was measured by qPCR analysis. The data showed that expression levels of specific genes were triggered 1 day after treatment. The expression levels of P450 subfamily genes, CYP306A1, CYP4G11 and CYP6AS14 were decreased in honey bee queens treated with low doses of coumaphos (5 ppm) and imidacloprid (0.02 ppm). Moreover, these two compounds suppressed the expression of genes related to antioxidation, immunity and development in queens at day 1. Up-regulation of antioxidants by these compounds in worker bees was observed at day 1. Coumaphos also caused a repression of CYP306A1 and CYP4G11 in workers. Antioxidants appear to prevent chemical damage to honey bees. We also found that DWV replication increased in workers treated with imidacloprid. This research clearly demonstrates that chemical exposure can affect sperm viability in queen honey bees. (C) 2016 Elsevier Ltd. All rights reserved.