Microwave cell death: Immunohistochemical and enzyme histochemical evaluation
Microwave cell death: Immunohistochemical and enzyme histochemical evaluation
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微波细胞死亡：免疫组织化学和酶组织化学评估
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
K. Kakudo
中科院分区：
文献类型：
--
作者：
T. Ozaki;I. Mori;Misa Nakamura;H. Utsunomiya;K. Tabuse;K. Kakudo
在日本，微波凝固疗法（MCT）已用于治疗原发性和转移性肝癌。在组织病理学检查中，病变的针吸活检检查经常显示有核癌细胞的存在，提示微波治疗不能完全消除癌细胞的结论，而计算机断层扫描和超声检查显示肿瘤消退。为了确定微波处理的组织是否含有功能活细胞，在微波处理的大鼠肝脏中使用免疫组织化学和酶组织化学方法检查Na+-K+-ATP酶蛋白及其活性。治疗后2天，微波探针周围出现4个同心的形态学可识别区域。A区位于最内侧海绵状区和外部坏死区之间，仅包含肝细胞的轻微形态学改变，其具有轻微的深染核和轻度嗜酸性细胞质。发现A区中的肝细胞的Na+-K+-ATP酶抗原性呈阳性，但酶活性呈阴性，表明A区正在发生细胞死亡，尽管在形态学上无法辨别。这种由微波处理引起的细胞死亡在形态上不同于先前已知的细胞死亡类型，无论是胀亡还是凋亡。
In Japan, microwave coagulation therapy (MCT) has been used for the management of primary and metastatic liver cancer. Needle biopsy examination from the lesion has frequently shown the presence of nucleated cancer cells in histopathological examinations, prompting the conclusion that cancer cells are not completely eliminated by microwave therapy, whereas computed tomography and ultrasonography examinations show tumor regression. To determine whether microwave‐treated tissue contains functionally viable cells, an examination of the Na+‐K+‐ATPase protein and its activity using immunohistochemical and enzyme histochemical methods were carried out in microwave‐treated rat liver. Four concentric, morphologically identifiable zones around the microwave probe needle appeared 2 days after treatment. Zone A, which was between the innermost spongy zone and the outer necrotic zone, contained only slight morphological alterations in the hepatocytes, which had slightly hyperchromatic nuclei and mildly eosinophilic cytoplasm. The hepatocytes in zone A were found to be positive for the Na+‐K+‐ATPase antigenicity but negative for enzyme activity, indicating that zone A was undergoing cell death, although morphologically this was not discernible. This type of cell death caused by microwave treatment is morphologically different from previously known types of cell death, either oncosis or apoptosis.
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电子显微镜的微波能量固定。
DOI：
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发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
Login,GR;Dvorak,AM
通讯作者：
Dvorak,AM
大鼠肝 (Na , K )-ATP 酶：通过免疫亲和层析分离 α 亚基并通过肽图谱进行结构分析。
影响因子：
2.9
作者：
Hubert,JJ;Schenk,DB;Skelly,H;Leffert,HL
通讯作者：
Leffert,HL
使用单克隆抗体定量正常和再生大鼠肝脏中的 (Na ,K )-ATP 酶活性和位点。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schenk,DB;Hubert,JJ;Leffert,HL
通讯作者：
Leffert,HL
肝 Na( )-K( )-ATP 酶活性与极化 β 亚基表达相关。
DOI：
10.1152/ajpcell.1995.269.1.c69
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Simon,FR;Leffert,HL;Ellisman,M;Iwahashi,M;Deerinck,T;Fortune,J;Morales,D;Dahl,R;Sutherland,E
通讯作者：
Sutherland,E
影响因子：
5.5
作者：
Li, F;Mao, HP;Borkan, SC
通讯作者：
Borkan, SC