Partial hepatectomy in the mouse: Technique and perioperative management
Partial hepatectomy in the mouse: Technique and perioperative management
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DOI：
10.1080/08941930390194424
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发表时间：
2003-03-01
影响因子：
1.9
通讯作者：
Puder, M
中科院分区：
文献类型：
--
作者：
Greene, AK;Puder, M
小鼠肝脏手术具有挑战性，因为肝脏的脆弱性，缺乏静脉通路和出血风险。为了研究手术切除后肝脏的再生能力，我们开发了一种新颖、快速、安全的小鼠部分肝切除技术。我们确定了小鼠肝脏七个叶的相对贡献，并切除了三个最前叶，以实现68%的肝切除。我们使用全身麻醉，一个小的上中线切口，丝线缝合要切除的肺叶，加温垫和灯，以及皮下注射生理盐水，以确保最小的发病率。我们在300只C57BL6小鼠中的288只（96%）中进行了安全的三分之二肝切除。围手术期死亡是由于技术错误。最低限度的长期发病率是赞赏。该技术可应用于小鼠的任何类型的肝切除。此外，这些小鼠的一般手术技术和围手术期管理可应用于用于外科研究的所有类型的小鼠腹腔内手术。
Hepatic surgery in mice is challenging because of the delicate nature of the liver, lack of intravenous access, and risk of hemorrhage. In order to study the ability of the liver to regenerate after surgical resection, we developed a novel, rapid, and safe technique for partial hepatectomy in mice. We determined the relative contributions of the seven lobes of the mouse liver and resected the three most anterior lobes for a 68% hepatectomy. We used general anesthesia, a small upper midline incision, silk suture to tie off the lobes to be resected, warming pads and lights, as well as subcutaneous saline injection to ensure minimal morbidity. We have performed a safe two-thirds hepatic resection in 288 of 300 C57BL6 mice (96%). Perioperative mortality was due to technical error. Minimal long-term morbidity was appreciated. This technique may be applied to any type of hepatic resection in mice. In addition, the general operative technique and perioperative management of these mice may be applied to all types of murine intra-abdominal procedures used for surgical research.