A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase.
A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase.
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来自植物病原真菌的抑制素可使编码苯丙氨酸解氨酶的植物防御基因的转录失活。
DOI：
10.1006/jmbi.1995.0313
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发表时间：
1995
影响因子：
5.6
通讯作者：
T. Yamada
中科院分区：
文献类型：
--
作者：
M. Wada;H. Kato;K. Malik;P. Sriprasertsak;Y. Ichinose;T. Shiraishi;T. Yamada
激发子和抑制素（抑制因子）都存在于豌豆病原菌松枝分枝杆菌孢子发芽液中。核运行试验表明，抑制素能迅速使豌豆上胚轴组织中苯丙氨酸解氨酶基因的激发子触发转录失活。研究了真菌病原菌分泌的信号分子使植物防御基因失活的机制。对豌豆苯丙氨酸解氨酶基因家族成员tata -近端抑制素响应的顺式作用序列和反式作用因子进行了体外研究。凝胶迁移分析和dna酶I足迹分析显示，PSPAL2的启动子区域被核因子在多个位点的结合所修饰，这些位点可能参与了抑制素介导的失活。在方框2和4以及近外显子序列上观察到抑制素的显著变化。
Both elicitor and supprescin (suppressor) are present in the pycnospore germination fluid of a pea pathogen Mycospharella pinodes. A nuclear run-on assay revealed that supprescin rapidly deactivated elicitor-triggered transcription of the gene encoding phenylalnine ammonia-lyase in pea epicotyl tissues. The mechanism underlying the deactivation of the plant defense gene by signal molecules secreted from the fungal pathogen was investigated. Cis-acting sequences and trans-acting factors responsive to supprescin in a TATA-proximal region of a member of the phenylalanine ammonia-lyase gene family in pea were examined in vitro. Gel mobility-shift assays and DNase I footprinting analysis revealed that the promoter region of PSPAL2 was modified by the binding of nuclear factors at multiple sites that were possibly involved in supprescin-mediated deactivation. The prominent changes by supprescin were observed at boxes 2 and 4 and near exonic sequences.