Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov.
Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov.
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DOI：
10.3389/fmicb.2019.02360
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发表时间：
2019-10-25
影响因子：
5.2
通讯作者：
Brisse, Sylvain
中科院分区：
文献类型：
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作者：
Merla, Cristina;Rodrigues, Carla;Brisse, Sylvain
产酸克雷伯氏菌引起人类机会性感染和抗生素后出血性腹泻。该肠杆菌科物种具有遗传异质性，目前细分为七个系统群（Ko1 至 Ko4 和 Ko6 至 Ko8）。在这里，我们研究了系统群 Ko3 和 Ko4 的分类地位。基于基因组序列的系统发育分析表明，Ko3 和 Ko4 形成了明确的序列簇，与密歇根克雷伯氏菌 (Ko1)、产酸克雷伯氏菌 (Ko2)、华西克雷伯氏菌 (Ko8) 和格里蒙氏克雷伯氏菌 (Ko6) 相关，但又不同。 Ko3和Ko4与K. huaxiensis和K. grimontii的平均核苷酸同一性(ANI)分别为90.7%和95.5%。此外，还从牛和人类粪便中分离出三种华西嵩草菌株，这是迄今为止基于中国尿路感染患者的单一菌株所描述的物种。生化和 MALDI-ToF 质谱分析可以将 Ko3、Ko4 和 Ko8 与其他产酸克氏杆菌物种区分开来。基于这些结果，我们建议将 Ko3 系统群命名为斯帕兰扎克雷伯氏菌 (Klebsiella spallanzanii)，以 SPARK_775_C1(T)（CIP 111695(T) 和 DSM 109531(T)）为模式菌株，将 Ko4 命名为巴氏克雷伯氏菌（Klebsiella pesturii），以 SPARK_836_C1(T)（CIP 111696(T) 和 DSM）作为模式菌株。 109530(T))作为类型菌株。从人类尿液、牛粪便和农场表面中分离出斯帕兰扎尼克鲁伯氏菌菌株，而在人类、牛和海龟的粪便中发现了巴斯德克鲁伯氏菌菌株。
Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1(T) (CIP 111695(T) and DSM 109531(T)) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1(T) (CIP 111696(T) and DSM 109530(T)) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.