Comprehensive studies on the DNA-binding and cleavage properties of a nickel complex derived from phthalate and 1,10-phenanthroline
Comprehensive studies on the DNA-binding and cleavage properties of a nickel complex derived from phthalate and 1,10-phenanthroline
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DOI：
10.1016/j.poly.2009.06.066
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发表时间：
2010-01
期刊：
影响因子：
2.6
通讯作者：
Qingxiang Wang;Wenqi Li;F. Gao;Shunxing Li;Jiancong Ni;Z. Zheng
中科院分区：
文献类型：
--
作者：
Qingxiang Wang;Wenqi Li;F. Gao;Shunxing Li;Jiancong Ni;Z. Zheng
通过电子吸收光谱、粘度、电化学、熔化温度和凝胶电泳测量全面研究了镍配合物 [Ni(phen)(pha)(H2O)3]（phen=1,10-菲咯啉，pha=邻苯二甲酸酯）与 DNA 的结合特性。与DNA相互作用后，[Ni(phen)(pha)(H2O)3]特征吸收峰的减色和红色显示出典型的插层现象，粘度和熔点实验证明了这一点。电化学分析表明，镍配合物插入DNA双螺旋结构后电化学活性被屏蔽。此外，琼脂糖凝胶电泳结果表明，在H2O2存在下，该复合物对超螺旋DNA具有中等的切割能力。
Binding properties of a nickel complex, [Ni(phen)(pha)(H2O)3] (phen=1,10-phenanthroline, pha=o-phthalate) to DNA were comprehensively investigated by electronic absorption spectroscopy, viscosity, electrochemistry, melting temperature and gel electrophoresis measurements. After interaction with DNA, the hypochromism and bathochromism for the characteristic absorption peaks of [Ni(phen)(pha)(H2O)3] revealed a typical intercalation, which was proved by viscosity and melting temperature experiments. Electrochemical assays showed that the electrochemical activity of the nickel complex was shielded after inserting into the double-helix structure of DNA. Moreover, the results of agarose gel electrophoresis showed that the complex had moderate cleavage ability to supercoiled DNA in the presence of H2O2.