Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.
Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.
复制标题
来自变形链球菌的葡聚糖结合蛋白基因在大肠杆菌中的表达。
DOI:
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发表时间:
1985
期刊:
影响因子:
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通讯作者:
G. Dougan
中科院分区:
文献类型:
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作者:
R. Russell;David C. Coleman;G. Dougan
The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage lambda vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.
DOI:
10.1099/00221287-128-5-1135
发表时间:
1982
期刊:
Journal of general microbiology
影响因子:
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作者:
Jagusztyn-Krynicka,EK;Smorawinska,M;Curtiss3rd,R
通讯作者:
Curtiss3rd,R