Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.

Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.
复制标题

来自变形链球菌的葡聚糖结合蛋白基因在大肠杆菌中的表达。

DOI:
--
复制
发表时间:
1985
期刊:
Journal of General Microbiology
影响因子:
--
通讯作者:
G. Dougan
G. Dougan
中科院分区:
--
文献类型:
--
作者:
R. Russell;David C. Coleman;G. Dougan

文献摘要

参考文献

被引文献

相似文献

变形链球菌的葡聚糖结合蛋白(GBP)的结构基因已被插入到λ噬菌体载体中,并在大肠杆菌K12中表达。E.用重组噬菌体感染的大肠杆菌含有与S.变异株GBP。在E.大肠杆菌中的果糖基转移酶可以在固定化葡聚糖上亲和纯化,并且针对它产生的抗血清已经显示出从S.变异人
The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage lambda vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.
克隆到质粒pBR322中的变形链球菌天冬氨酸半醛脱氢酶基因的表达。
DOI: 10.1099/00221287-128-5-1135
发表时间: 1982
期刊: Journal of general microbiology
影响因子: --
作者:
Jagusztyn-Krynicka,EK;Smorawinska,M;Curtiss3rd,R
通讯作者: Curtiss3rd,R