Big angiotensin-25 : A novel glycosylated angiotensin-related peptide isolated from human urine
Big angiotensin-25 : A novel glycosylated angiotensin-related peptide isolated from human urine
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Big angiotensin-25：从人尿中分离出的一种新型糖基化血管紧张素相关肽
DOI：
10.1016/j.bbrc.2013.10.124
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kitamura K
中科院分区：
文献类型：
--
作者：
Nagata S;Hatakeyama K;Asami M;Tokashiki M;Hibino H;Nishiuchi Y;Kuwasako K;Kato J;Asada Y;Kitamura K
肾素-血管紧张素系统(RAS)，包括血管紧张素Ⅱ(Ang II)，在调节血压和体液平衡方面起着重要作用。因此，RAS已成为治疗肾脏和心血管疾病的关键靶点。在使用抗血管紧张素II N端部分的抗体寻找生物活性多肽的过程中，我们从人尿中鉴定并表征了一种新的血管紧张素相关肽作为主要的分子形式。我们将该肽命名为大血管紧张素-25(Bang-25)，因为它由25个氨基酸组成，带有一个糖链，并添加了半胱氨酸。Bang-25可被糜酶迅速切割成Ang II，但不能被肾素切割。这种多肽在人体尿液中含量丰富，存在于各种器官和组织中。特别是，Bang-25在肾脏中的免疫染色专门定位于足细胞。尽管Bang-25的生理功能仍不确定，但我们的发现表明，它是由血管紧张素原加工而成的，可能代表着组织中Ang II合成的另一种不依赖肾素的途径。
The renin–angiotensin system (RAS), including angiotensin II (Ang II), plays an important role in the regulation of blood pressure and body fluid balance. Consequently, the RAS has emerged as a key target for treatment of kidney and cardiovascular disease. In a search for bioactive peptides using an antibody against the N-terminal portion of Ang II, we identified and characterized a novel angiotensin-related peptide from human urine as a major molecular form. We named the peptide Big angiotensin-25 (Bang-25) because it consists of 25 amino acids with a glycosyl chain and added cysteine. Bang-25 is rapidly cleaved by chymase to Ang II, but is resistant to cleavage by renin. The peptide is abundant in human urine and is present in a wide range of organs and tissues. In particular, immunostaining of Bang-25 in the kidney is specifically localized to podocytes. Although the physiological function of Bang-25 remains uncertain, our findings suggest it is processed from angiotensinogen and may represent an alternative, renin-independent path for Ang II synthesis in tissue.