CORONAVIRUS SPECIES SPECIFICITY - MURINE CORONAVIRUS BINDS TO A MOUSE-SPECIFIC EPITOPE ON ITS CARCINOEMBRYONIC ANTIGEN-RELATED RECEPTOR GLYCOPROTEIN
CORONAVIRUS SPECIES SPECIFICITY - MURINE CORONAVIRUS BINDS TO A MOUSE-SPECIFIC EPITOPE ON ITS CARCINOEMBRYONIC ANTIGEN-RELATED RECEPTOR GLYCOPROTEIN
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DOI：
10.1128/jvi.66.12.7420-7428.1992
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发表时间：
1992-12-01
影响因子：
5.4
通讯作者：
HOLMES, KV
中科院分区：
文献类型：
--
作者：
COMPTON, SR;STEPHENSEN, CB;HOLMES, KV
像大多数冠状病毒一样，冠状病毒小鼠肝炎病毒(MHV)表现出很强的物种特异性，只在小鼠中引起自然感染。MHV-A59病毒粒子使用癌胚抗原(CEA)家族中110-120 kDa的糖蛋白(MHVR)作为受体(G.S.Dveksler，M.N.Pensiero，C.B.Cardellichio，R.K.Williams，G.S.酱，K.V.Holmes和C.W.Dieffenbach，J.Virol)。65：6881-6891,1991；R.K.Williams，G.S.酱，K.V.Holmes，Proc.娜塔莉。阿卡德。SCI。美国88：5533-5536,1991)。通过比较病毒和抗受体抗体与多种细胞系和肠道刷状缘膜(BBM)的结合，探讨病毒-受体相互作用在确定MHV-A59种属特异性中的作用。用BALB/c BBM免疫SJL/J小鼠所产生的多克隆抗受体抗血清(抗MHVR)可识别BALB/c BBM免疫印记中的MHVR，但不识别成年SJL/J小鼠抗MHV-A59感染的BBM中的MHVR，这表明MHVR与其不结合MHV(7)的SJL/J同源基因在表位上存在很大差异。抗MHVR结合到MHV敏感小鼠细胞系的质膜上，但不结合到人、猫、狗、猴或仓鼠细胞系的质膜上。这些物种的细胞系对MHV-A59感染具有抵抗力，只有被测试的小鼠细胞系对MHV-A59感染敏感。用抗MHVR对小鼠成纤维细胞进行预处理可防止放射性标记的病毒粒子与小鼠细胞结合，并防止病毒感染。固相病毒结合试验和病毒重叠蛋白印迹试验表明，MHV-A59病毒粒子可与MHV易感小鼠的肠道BBM上的MHVR结合，但不能与人、猫、狗、猪、牛、兔、大鼠、棉鼠或鸡的BBM上的蛋白质结合。在这些物种的BBM免疫印迹中，阻断MHV-A59感染小鼠细胞的多克隆和单克隆抗受体抗体只识别小鼠CEA相关糖蛋白，而不识别其他物种的同源CEA相关糖蛋白。这些结果表明，MHV-A59与MHVR的小鼠特异性表位结合，支持MHV-A59感染的物种特异性可能是由于病毒-受体相互作用的特异性。
Like most coronaviruses, the coronavirus mouse hepatitis virus (MHV) exhibits strong species specificity, causing natural infection only in mice. MHV-A59 virions use as a receptor a 110- to 120-kDa glycoprotein (MHVR) in the carcinoembryonic antigen (CEA) family of glycoproteins (G. S. Dveksler, M. N. Pensiero, C. B. Cardellichio, R. K. Williams, G. S. Jiang, K. V. Holmes, and C. W. Dieffenbach, J. Virol. 65:6881-6891, 1991; and R. K. Williams, G. S. Jiang, and K. V. Holmes, Proc. Natl. Acad. Sci. USA 88:5533-5536, 1991). The role of virus-receptor interactions in determining the species specificity of MHV-A59 was examined by comparing the binding of virus and antireceptor antibodies to cell lines and intestinal brush border membranes (BBM) from many species. Polyclonal antireceptor antiserum (anti-MHVR) raised by immunization of SJL/J mice with BALB/c BBM recognized MHVR specifically in immunoblots of BALB/c BBM but not in BBM from adult SJL/J mice that are resistant to infection with MHV-A59, indicating a major difference in epitopes between MHVR and its SJL/J homolog which does not bind MHV (7). Anti-MHVR bound to plasma membranes of MHV-susceptible murine cell lines but not to membranes of human, cat, dog, monkey, or hamster cell lines. Cell lines from these species were resistant to MHV-A59 infection, and only the murine cell lines tested were susceptible. Pretreatment of murine fibroblasts with anti-MHVR prevented binding of radiolabeled virions to murine cells and prevented virus infection. Solid-phase virus-binding assays and virus overlay protein blot assays showed that MHV-A59 virions bound to MHVR on intestinal BBM from MHV-susceptible mouse strains but not to proteins on intestinal BBM from humans, cats, dogs, pigs, cows, rabbits, rats, cotton rats, or chickens. In immunoblots of BBM from these species, both polyclonal and monoclonal antireceptor antibodies that block MHV-A59 infection of murine cells recognized only the murine CEA-related glycoprotein and not homologous CEA-related glycoproteins of other species. These results suggest that MHV-A59 binds to a mouse-specific epitope of MHVR, and they support the hypothesis that the species specificity of MHV-A59 infection may be due to the specificity of the virus-receptor interaction.