The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes
The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes
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DOI：
10.1016/j.cropro.2007.06.008
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发表时间：
2008-03-01
期刊：
影响因子：
2.8
通讯作者：
Gowen, S. R.
中科院分区：
文献类型：
--
作者：
Anastasiadis, I. A.;Giannakou, I. O.;Gowen, S. R.
在盆栽和温室实验中评价了含有天然存在的真菌淡紫拟青霉（Paecilomyces lilacinus）菌株251的孢子的配制产品对根结线虫的有效性。使用剂量为4千克/公顷的生物杀线虫剂后，从卵中孵化出的第二阶段幼鱼减少，而剂量加倍后则进一步减少。然而，死亡率下降，增加接种量。盆栽试验表明，淡紫拟青霉和坚强芽孢杆菌单独或联合使用，对根结线虫二龄幼虫、卵或卵块均有较好的防治效果。在温室实验中，评价了生物杀线虫剂作为单独方法或与土壤日晒结合控制根结线虫的潜力。将土壤日晒15 d，在除去塑料片后立即施用生物杀线虫剂。单独或结合使用淡紫青霉进行15 d的土壤日晒处理对根结线虫的防治效果均不令人满意。在移植前2周和移植过程中施用杀线威，得到与含有淡紫青霉的商业产品类似的结果，但上级土壤日晒。(C)2007爱思唯尔有限公司版权所有。
The effectiveness of a formulated product containing spores of the naturally occurring fungus Paecilomyces lilacinus, strain 251, was evaluated against root-knot nematodes in pot and greenhouse experiments. Decrease of second-stage juveniles hatching from eggs was recorded by using the bio-nematicide at a dose of 4 kg ha(-1), while a further decrease was recorded by doubling the dose. However, the mortality rate decreased by increasing the inoculum level. Application of P. lilacinus and Bacillus firmus, singly or together in pot experiments, provided effective control of second-stage juveniles, eggs or egg masses of root-knot nematodes. In a greenhouse experiment, the bio-nematicide was evaluated for its potential to control root-knot nematodes either as a stand-alone method or in combination with soil solarization. Soil was solarized for 15 d and the bio-nematicide was applied just after the removal of the plastic sheet. Soil solarization for 15 d either alone or combined with the use of P. lilacinus did not provide satisfactory control of root-knot nematodes. The use of oxamyl, which was applied 2 weeks before and during transplanting, gave results similar to the commercial product containing P. lilacinus but superior to soil solarization. (C) 2007 Elsevier Ltd. All rights reserved.