COMPARISON OF TOXICITY BETWEEN POPULATIONS OF GONYAULAX-TAMARENSIS OF EASTERN NORTH-AMERICAN WATERS
COMPARISON OF TOXICITY BETWEEN POPULATIONS OF GONYAULAX-TAMARENSIS OF EASTERN NORTH-AMERICAN WATERS
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DOI：
10.1016/0272-7714(85)90020-4
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发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
SHIMIZU, Y
中科院分区：
文献类型：
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作者：
MARANDA, L;ANDERSON, DM;SHIMIZU, Y
从沿着西北大西洋沿岸从加拿大芬迪湾到美国长岛采集的底栖孢囊或游动细胞中分离出塔玛膝沟藻。所有克隆在相同条件下生长，并在盲法试验中测定毒素含量和组成。发现毒素含量（μ小鼠单位/细胞）的差异跨越两个数量级，毒性从北到南递减。一些分离株的毒素水平检测不到。南方株G. tamarensis可能解释了麻痹性贝类中毒（PSP）的历史上没有在囊肿和运动细胞已报告的地区。观察到的地理模式的原因是未知的，但它确实表明，可能有一个环境确定的南部限制的区域PSP的问题。一些克隆的毒素组成的定性数据表明，石房蛤毒素，neosaxitoxin和gonyautoxins-II，-III和-IV的普遍存在。需要一种更定量的方法（即检查每个分离株的所有12种膝沟藻毒素），以充分利用毒素组成的潜力来区分菌株。
Isolates of the dinoflagellate Gonyaulax tamarensis were established from benthic cysts or motile cells collected along the north-western Atlantic coast from the Bay of Fundy [Canada] to Long Island [USA]. All clones were grown under the same conditions and assayed in a blind test for toxin content and composition. Differences in toxin content (.mu.mouse unit per cell) spanning two orders of magnitude were found, with decreasing toxicity from north to south. Some isolates had undetectable toxin levels. The low toxicity of southern strains of G. tamarensis may explain the historical absence of paralytic shellfish poisoning (PSP) in areas where cysts and motile cells have been reported. The cause of the observed geographic pattern is unknown, but it does suggest that there may be an environmentally-determined southern limit to the regional PSP problem. Qualitative data on the toxin composition of some clones indicate that saxitoxin, neosaxitoxin and gonyautoxins-II, -III and -IV are generally present. A more quantitative approach (i.e. one which examines each isolate for all of the 12 Gonyaulax toxins) is needed to fully utilize the potential of toxin composition in discriminating between strains.