Changes in the expression of corticotrophin-releasing hormone, mineralocorticoid receptor and glucocorticoid receptor mRNAs in the hypothalamic paraventricular nucleus induced by fornix transection and adrenalectomy
Changes in the expression of corticotrophin-releasing hormone, mineralocorticoid receptor and glucocorticoid receptor mRNAs in the hypothalamic paraventricular nucleus induced by fornix transection and adrenalectomy
复制标题
穹窿横断和肾上腺切除所致下丘脑室旁核促肾上腺皮质激素释放激素、盐皮质激素受体和糖皮质激素受体mRNA表达的变化
DOI：
10.1111/j.1365-2826.2006.01519.x
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发表时间：
2007-04-01
影响因子：
3.2
通讯作者：
Kawata, M.
中科院分区：
文献类型：
--
作者：
Han, F.;Ozawa, H.;Kawata, M.
下丘脑室旁核（PVN）接受来自海马的输入。本研究探讨了海马对PVN糖皮质激素反馈基因的影响。因此，在穹窿横断的大鼠中，通过原位杂交检测PVN中促肾上腺皮质激素释放激素（CRH）、盐皮质激素受体（MR）和糖皮质激素受体（GR）的mRNA表达。穹窿横断（FT）后，在小细胞PVN中观察到CRH、MR和GR mRNA的显著增加。FT动物肾上腺切除术也显示CRH和GR mRNA阳性细胞数量增加。双侧肾上腺切除后CRH、MR和GR mRNA的表达也增加，FT横断动物小细胞PVN中GR mRNA的表达进一步增强。然而，在大细胞PVN中没有明显的这种变化。这些结果表明，从海马的PVN，特别是其小细胞区域的输入，MR，GR和CRH的mRNA的表达，可能独立于皮质酮的反馈作用，具有独特的和差异的抑制作用。
The paraventricular nucleus (PVN) in the hypothalamus receives inputs from the hippocampus. The present study explored the influence of the hippocampus on genes mediating glucocorticoid feedback in the PVN. Accordingly, the expression of mRNAs for corticotrophin-releasing hormone (CRH), the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR) in the PVN was examined by in situ hybridisation in rats subjected to transection of the fornix. Significant increases in CRH, MR and GR mRNAs were observed in the parvocellular PVN after fornix transection (FT). FT-animals subjected to adrenalectomy also showed an increase in the number of cells positive for CRH and GR mRNAs. CRH, MR and GR mRNA expression was also increased by bilateral adrenalectomy, and GR mRNA expression was further enhanced in the parvocellular PVN of the FT transected animals. However, no such changes were evident in the magnocellular PVN. These results suggest that the input from the hippocampus to the PVN, particularly to its parvocellular region, has distinct and differential inhibitory effects on the expression of MR, GR and CRH mRNAs that may operate independently from the feedback actions of corticosterone.